Hough, Barbara R. and Yancey, Paul H. and Davidson, Eric H. (1973) Persistence of maternal RNA in Engystomops embryos. Journal of Experimental Zoology, 185 (3). pp. 357-368. ISSN 0022-104X. doi:10.1002/jez.1401850310. https://resolver.caltech.edu/CaltechAUTHORS:20151216-165729835
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Abstract
Females of the neotropic anuran Engystomops pustulosus were injected with 3H-uridine and produced eggs containing RNA labeled during synchronous oogenesis. The >4S, labeled RNA extracted from these eggs, and from embryos which developed from them, was hybridized with an isolated repetitive fraction of Engystomops DNA, and the hybrids were assayed on hydroxyapatite columns. About 1% of the egg RNA is transcribed from DNA sequences included in this fraction. Hybridization of labeled ribosomal RNA was minimized by the addition of excess unlabeled ribosomal RNA extracted from Xenopus ovaries. The labeled RNA of embryos up to the tadpole stage was identified as maternal transcript by competition experiments with unlabeled Engystomops oocyte RNA. It was found that maternally-labeled RNA is retained in embryos at least up to the tadpole stage.
Item Type: | Article | |||||||||
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Additional Information: | © 1973 Wiley-Liss, Inc. This research was supported by the National Institute of Child Health and Development grant HD-05753, and National Science Foundation grant GB-33441X. We acknowledge the expert technical assistance of Ms. Margaret Chemberlin, and of Ms. Jane Rigg and Mr. Charles Collett in the care of the Engystomops colony and collection of embryos. Special thanks are due to Drs. Roy J. Britten and Michael J. Smith for their invaluable assistance in the collection of toads in Panama. | |||||||||
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Issue or Number: | 3 | |||||||||
DOI: | 10.1002/jez.1401850310 | |||||||||
Record Number: | CaltechAUTHORS:20151216-165729835 | |||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20151216-165729835 | |||||||||
Official Citation: | Hough, B. R., Yancey, P. H. and Davidson, E. H. (1973), Persistence of maternal RNA in Engystomops embryos. J. Exp. Zool., 185: 357–368. doi: 10.1002/jez.1401850310 | |||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
ID Code: | 63008 | |||||||||
Collection: | CaltechAUTHORS | |||||||||
Deposited By: | George Porter | |||||||||
Deposited On: | 27 Jan 2016 23:53 | |||||||||
Last Modified: | 10 Nov 2021 23:10 |
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