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DNA sequence organization in the genomes of five marine invertebrates

Goldberg, Robert B. and Crain, William R. and Ruderman, Joan V. and Moore, Gordon P. and Barnett, Thomas R. and Higgins, Ratchford C. and Gelfand, Robert A. and Galau, Glenn A. and Britten, Roy J. and Davidson, Eric H. (1975) DNA sequence organization in the genomes of five marine invertebrates. Chromosoma, 51 (3). pp. 225-251. ISSN 0009-5915. http://resolver.caltech.edu/CaltechAUTHORS:20151216-170610841

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Abstract

The arrangement of repetitive and non-repetitive sequence was studied in the genomic DNA of the oyster (Crassostrea virginica), the surf clam (Spisula solidissima), the horseshoe crab (Limulus polyphemus), a nemertean worm (Cerebratulus lacteus) and a jellyfish (Aurelia aurita). Except for the jellyfish these animals belong to the protostomial branch of animal evolution, for which little information regarding DNA sequence organization has previously been available. The reassociation kinetics of short (250–300 nucleotide) and long (2,000–3,000 nucleotide) DNA fragments was studied by the hydroxyapatite method. It was shown that in each case a major fraction of the DNA consists of single copy sequences less than about 3,000 nucleotides in length, interspersed with short repetitive sequences. The lengths of the repetitive sequences were estimated by optical hyperchromicity and S1 nuclease measurements made on renaturation products. All the genomes studied include a prominent fraction of interspersed repetitive sequences about 300 nucleotides in length, as well as longer repetitive sequence regions.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1007/BF00284817DOIArticle
http://link.springer.com/article/10.1007/BF00284817PublisherArticle
Additional Information:© 1975 Springer. Received and accepted April 11, 1975 by J. G. Gall. Ready for press April 16, 1975. We are pleased to acknowledge the expert technical assistance of Ms. Margaret Chamberlin and Ms. Denise Painchaud. This research was supported by NIH Training Grant No. HD-00014 to the Marine Biological Laboratory's Embryology Course and by NIH Grant No. GM-20927.
Funders:
Funding AgencyGrant Number
NIHHD-00014
NIHGM-20927
Record Number:CaltechAUTHORS:20151216-170610841
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20151216-170610841
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63009
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:19 Jan 2016 21:56
Last Modified:13 Feb 2019 18:06

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