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Single copy DNA and structural gene sequence relationships among four sea urchin species

Angerer, Robert C. and Davidson, Eric H. and Britten, Roy J. (1976) Single copy DNA and structural gene sequence relationships among four sea urchin species. Chromosoma, 56 (3). pp. 213-226. ISSN 0009-5915. https://resolver.caltech.edu/CaltechAUTHORS:20151216-172028874

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Abstract

Measurements of the divergence of single copy DNA sequences among four sea urchin species are presented. At a standard criterion for reassociation (0.12 M phosphate buffer, 60° C, hydroxyapatite binding) we observe the following extents of reaction and reductions in thermal stability for single copy DNA reassociation between Strongylocentrotus purpuratus tracer and heterologous driver DNA: S. dröbachiensis 68% and 2.5°C; S. franciscanus 51% and 3.5° C; Lytechinus pictus 12% and 7.5° C. The implied extents of sequence relatedness are consistent with the phylogenetic relationships of these species. The rate of single copy sequence divergence in the evolutionary lines leading to the Strongylocentrotus species is estimated to be 0.06–0.35% per million years. The rate of divergence of total single copy sequence has been compared to that of structural gene sequences represented in S. purpuratus gastrula polysomal messenger RNA. When closely related species, S. purpuratus and S. franciscanus, are compared, these polysomal sequences are found to diverge at a lower rate than does the total single copy sequence. For two very distantly related species, S. purpuratus and L. pictus, a small fraction of the single copy DNA sequence is probably conserved. These conserved sequences are not enriched in their content of structural gene sequences.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1007/BF00293186DOIArticle
Additional Information:© 1976 Springer. Received March 10, 1976. Accepted March 22, 1976 by J. G. Gall. Ready for press March 29, 1976. This research was supported by grants from the National Institutes of Health #HD-05753 and #GM-20927 and by a grant from the National Science Foundation #BMS75-07359. R.C.A. was the recipient of fellowships from the American Cancer Society, Grant #PF-925, and from the American Cancer Society, California Division, Grant #J-309.
Funders:
Funding AgencyGrant Number
NIHHD-05753
NIHGM-20927
NSFBMS75-07359
American Cancer SocietyPF-925
American Cancer Society, California DivisionJ-309
Issue or Number:3
Record Number:CaltechAUTHORS:20151216-172028874
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20151216-172028874
Official Citation:Angerer, R.C., Davidson, E.H. & Britten, R.J. Single copy DNA and structural gene sequence relationships among four sea urchin species. Chromosoma 56, 213–226 (1976). https://doi.org/10.1007/BF00293186
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63011
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:21 Jan 2016 21:47
Last Modified:12 Oct 2020 20:51

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