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Absence of short period interspersion of repetitive and non-repetitive sequences in the DNA of Drosophila melanogaster

Crain, William R. and Eden, Francine C. and Pearson, William R. and Davidson, Eric H. and Britten, Roy J. (1976) Absence of short period interspersion of repetitive and non-repetitive sequences in the DNA of Drosophila melanogaster. Chromosoma, 56 (4). pp. 309-326. ISSN 0009-5915. https://resolver.caltech.edu/CaltechAUTHORS:20151216-172238139

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Abstract

A sensitive search has been made in Drosophila melanogaster DNA for short repetitive sequences interspersed with single copy sequences. Five kinds of measurements all yield the conclusion that there are few short repetitive sequences in this genome: 1) Comparison of the kinetics of reassociation of short (360 nucleotide) and long (1,830 nucleotide) fragments of DNA; 2) reassociation kinetics of long fragments (2,200 nucleotide) with an excess of short (390 short nucleotide) fragments; 3) measurement of the size of S1 nuclease resistant reassociated repeated sequences; 4) measurement of the hyperchromicity of reassociated repetitive fragments as a function of length; 5) direct assay by kinetics of reassociation of the amount of single copy sequence present on 1,200 nucleotide long fragments which also contain repetitive sequences.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1007/BF00292953DOIArticle
Additional Information:© 1976 Springer. Received March 2, 1976. Accepted March 22, 1976 by J. G. Gall. Ready for press March 30, 1976. We thank Dr. Ray White for his generous gift of tritium labeled Drosophila DNA. This work was supported by grants from the National Institutes of Health (HD-05753 and GM-20927) and from the National Science Foundation (BMS75-07359). W.R.C. holds a postdoctoral fellowship from the National Institutes of Health (GM-55726). F.C.E. held a postdoctoral fellowship from the American Cancer Society (PF-955). W.R.P. is a predoctoral fellow on a National Institutes of Health tranining grant (GM-00086).
Funders:
Funding AgencyGrant Number
NIHHD-05753
NIHGM-20927
NSFBMS75-07359
NIH Postdoctoral FellowshipGM-55726
American Cancer SocietyPF-955
NIH Predoctoral FellowshipGM-00086
Issue or Number:4
Record Number:CaltechAUTHORS:20151216-172238139
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20151216-172238139
Official Citation:Crain, W.R., Eden, F.C., Pearson, W.R. et al. Absence of short period interspersion of repetitive and non-repetitive sequences in the DNA of Drosophila melanogaster . Chromosoma 56, 309–326 (1976). https://doi.org/10.1007/BF00292953
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ID Code:63012
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:21 Jan 2016 21:09
Last Modified:12 Oct 2020 20:55

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