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A large-scale laboratory maintenance system for gravid purple sea urchins (Strongylocentrotus purpuratus)

Leahy, Patrick S. and Tutschulte, Theodore C. and Britten, Roy J. and Davidson, Eric H. (1978) A large-scale laboratory maintenance system for gravid purple sea urchins (Strongylocentrotus purpuratus). Journal of Experimental Zoology, 204 (3). pp. 369-380. ISSN 0022-104X. https://resolver.caltech.edu/CaltechAUTHORS:20151217-174102169

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Abstract

A large-scale laboratory facility for the maintenance of several thousand gravid sea urchins (S. purpuratus) is described. Gametes of this species provide an important research resource for studies of animal development. Methods are described for culturing adult S. purpuratus for periods up to two years or more with almost no mortality after the first three to four weeks following collection. Adult females can be maintained in a fertile state in the culture system for four to six months. When spawned, gravid females living in the culture facility will routinely regenerate normal sized populations of fertile oocytes at 1 to 2-month intervals. Such females can be used repeatedly as a source of mature gametes for laboratory research. The reproductive performance of these females can be predicted approximately by the number of late vitellogenic oocytes present in their ovaries. After several months the pool of these oocytes is exhausted and no further mature oocytes can be found for a long period. We show, however, that such females are able to carry out a complete annual cycle of oogenesis if held for a long enough period of time in the culture system.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/jez.1402040308DOIArticle
http://onlinelibrary.wiley.com/doi/10.1002/jez.1402040308/abstractPublisherArticle
Additional Information:© 1978 Wiley-Liss. The authors wish to acknowledge financial support from the South Dakota State University Agricultural Experiment Station (SD 737), National Institutes of Health (HD 08631), and the Lalor Foundation. This manuscript has been approved for publication as Journal Series No. 1506 by the Director, Agricultural Experiment Station, South Dakota State University.
Funders:
Funding AgencyGrant Number
South Dakota State UniversitySD 737
NIHHD 08631
Lalor FoundationUNSPECIFIED
Issue or Number:3
Record Number:CaltechAUTHORS:20151217-174102169
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20151217-174102169
Official Citation:Leahy, P. S., Tutschulte, T. C., Britten, R. J. and Davidson, E. H. (1978), A large-scale laboratory maintenance system for gravid purple sea urchins (Strongylocentrotus purpuratus). J. Exp. Zool., 204: 369–380. doi: 10.1002/jez.1402040308
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63047
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:27 Jan 2016 23:52
Last Modified:03 Oct 2019 09:24

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