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In vivo competition identifies positive cis-regulatory elements required for lineage-specific gene expression in the sea urchin embryo

Franks, Roberta R. and Britten, Roy J. and Davidson, Eric H. (1989) In vivo competition identifies positive cis-regulatory elements required for lineage-specific gene expression in the sea urchin embryo. In: Cellular Basis of Morphogenesis. Ciba Foundation Symposium. No.144. Wiley , Chichester, UK, 156-66; discussion 166. ISBN 9780471923060.

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Several cis-regulatory elements within the 5' regulatory region of the lineage-specific CyIIIa actin gene have been identified by in vivo competition. Sea urchin eggs were coinjected with a fusion construct in which the bacterial chloramphenicol acetyltransferase (CAT) gene is controlled by the CyIIIa regulatory domain, together with molar excesses of various DNA subfragments that are derived from this region. Each subfragment studied includes one or several known sites where highly specific interactions occur in vitro with nuclear DNA-binding proteins. Coinjection of excess molecules of some of these subregions results in a decrease in the activity of the CyIIIa-CAT fusion gene, as a function of the molar subfragment: CyIIIa-CAT ratio. This result implies that these sites complete with cis sequences linked to the CAT reporter gene for limited factors that positively regulate CyIIIa transcription in the embryo, and demonstrates the functional importance of a number of the DNA-protein interactions that have been observed in vitro.

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Additional Information:© Ciba Foundation 1989. Research was supported by NIH grant HD-05753.
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Subject Keywords:gene expression; embryogenesis; DNA-binding; CAT reporter; cell lineage
Series Name:Ciba Foundation Symposium
Issue or Number:144
Record Number:CaltechAUTHORS:20151217-185657663
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Official Citation:Franks, R. R., Britten, R. J. and Davidson, E. H. (2007) In Vivo Competition Identifies Positive Cis-Regulatory Elements Required for Lineage-Specific Gene Expression in the Sea Urchin Embryo, in Ciba Foundation Symposium 144 - Cellular Basis of Morphogenesis (eds D. Evered and J. Marsh), John Wiley & Sons, Ltd., Chichester, UK. doi: 10.1002/9780470513798.ch9
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63054
Deposited By: George Porter
Deposited On:25 Feb 2016 23:39
Last Modified:10 Nov 2021 23:11

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