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Automated sequential affinity chromatography of sea urchin embryo DNA binding proteins

Coffman, J. A. and Moore, J. G. and Calzone, F. J. and Britten, R. J. and Hood, L. E. and Davidson, E. H. (1992) Automated sequential affinity chromatography of sea urchin embryo DNA binding proteins. Molecular Marine Biology and Biotechnology, 1 (2). pp. 136-46. ISSN 1053-6426.

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An automated method of running a tandem sequence of oligonucleotide affinity columns was used to purify factors that interact specifically with cis-regulatory sites of the CyIIIa cytoskeletal actin gene of the sea urchin embryo (Strongylocentrotus purpuratus). The method allows quantitative enrichment in a single chromatographic run of up to 12 different sequence-specific DNA binding proteins, each of which may then be readily purified to homogeneity by methods such as preparative gel electrophoresis. The affinity chromatography and identification of six different CyIIIa-regulatory factors is described, and the general utility of the method is discussed.

Item Type:Article
Hood, L. E.0000-0001-7158-3678
Additional Information:© 1992 Springer.
Issue or Number:2
Record Number:CaltechAUTHORS:20151218-080949915
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63057
Deposited By: George Porter
Deposited On:24 Feb 2016 23:01
Last Modified:03 Oct 2019 09:24

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