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Calculation of sequence divergence from the thermal stability of DNA heteroduplexes

Springer, Mark S. and Davidson, Eric H. and Britten, Roy J. (1992) Calculation of sequence divergence from the thermal stability of DNA heteroduplexes. Journal of Molecular Evolution, 34 (5). pp. 379-382. ISSN 0022-2844. doi:10.1007/BF00162994.

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Measurements are reported of the thermal stability of DNA heteroduplexes between clones of the eta-globin pseudogene from a variety of primates. The known sequences of this 7.1-kb region differ from each over a range from 1.6% for human versus chimp to nearly 12% for human versus spider monkey. Thermal stability was determined by standard hydroxyapatite thermal elution, and the results show a precisely linear decrease in thermal stability with divergence. The slope of the regression line is 1.18% sequence divergence per degree centigrade reduction in thermal stability.

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Additional Information:© 1992 Springer. Received September 30, 1991 / Accepted December 3, 1991. We thank Jeffrey R. Powell for helpful comments on an earlier draft of this manuscript. M.S. was supported by N.I.H. Training Grant HD-07257. This work was supported by a Developmental Biology Grant from the Lucille P. Markey Charitable Trust.
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Lucille P. Markey Charitable TrustUNSPECIFIED
Subject Keywords:DNA/DNA hybridization; ΔTm; Hydroxyapatite
Issue or Number:5
Record Number:CaltechAUTHORS:20151218-081157341
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63058
Deposited By: George Porter
Deposited On:29 Jan 2016 20:46
Last Modified:10 Nov 2021 23:11

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