Springer, Mark S. and Davidson, Eric H. and Britten, Roy J. (1992) Calculation of sequence divergence from the thermal stability of DNA heteroduplexes. Journal of Molecular Evolution, 34 (5). pp. 379-382. ISSN 0022-2844. https://resolver.caltech.edu/CaltechAUTHORS:20151218-081157341
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Abstract
Measurements are reported of the thermal stability of DNA heteroduplexes between clones of the eta-globin pseudogene from a variety of primates. The known sequences of this 7.1-kb region differ from each over a range from 1.6% for human versus chimp to nearly 12% for human versus spider monkey. Thermal stability was determined by standard hydroxyapatite thermal elution, and the results show a precisely linear decrease in thermal stability with divergence. The slope of the regression line is 1.18% sequence divergence per degree centigrade reduction in thermal stability.
| Item Type: | Article | |||||||||
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| Additional Information: | © 1992 Springer. Received September 30, 1991 / Accepted December 3, 1991. We thank Jeffrey R. Powell for helpful comments on an earlier draft of this manuscript. M.S. was supported by N.I.H. Training Grant HD-07257. This work was supported by a Developmental Biology Grant from the Lucille P. Markey Charitable Trust. | |||||||||
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| Subject Keywords: | DNA/DNA hybridization; ΔTm; Hydroxyapatite | |||||||||
| Issue or Number: | 5 | |||||||||
| Record Number: | CaltechAUTHORS:20151218-081157341 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20151218-081157341 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 63058 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | George Porter | |||||||||
| Deposited On: | 29 Jan 2016 20:46 | |||||||||
| Last Modified: | 03 Oct 2019 09:24 |
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