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The Garz Sec7 domain guanine nucleotide exchange factor for Arf regulates salivary gland development in Drosophila

Szul, Tomasz and Burgess, Jason and Jeon, Mili and Zinn, Kai and Marques, Guillermo and Brill, Julie A. and Sztul, Elizabeth (2011) The Garz Sec7 domain guanine nucleotide exchange factor for Arf regulates salivary gland development in Drosophila. Cellular Logistics, 1 (2). pp. 69-76. ISSN 2159-2799. PMCID PMC3116586. https://resolver.caltech.edu/CaltechAUTHORS:20151218-090549591

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Abstract

Surface delivery of proteins involved in cell-cell and cell-matrix interactions in cultured mammalian cells requires the GBF1 guanine nucleotide exchange factor. However, the role of GBF1 in delivery of adhesion proteins during organogenesis in intact animals has not been characterized. Here, we report the function of the fly GBF1 homolog, Gartenzwerg (Garz) in the development of the salivary gland in Drosophila melanogaster. We used the GAL4/UAS system to selectively deplete Garz from salivary gland cells. We show that depletion of Garz disrupts the secretory pathway as evidenced by the collapse of Golgi-localized Lava lamp (Lva) and the TGN-localized gamma subunit of the clathrin-adaptor protein complex (AP-1). Additionally, Garz depletion inhibits trafficking of cell-cell adhesion proteins cadherin (DE-cad) and Flamingo to the cell surface. Disregulation of trafficking correlates with mistargeting of the tumor suppressor protein Discs large involved in epithelial polarity determination. Garz-depleted salivary cells are smaller and lack well-defined plasma membrane domains. Garz depletion also inhibits normal elongation and positioning of epithelial cells, resulting in a disorganized salivary gland that lacks a well defined luminal duct. Our findings suggest that Garz is essential for establishment of epithelial structures and demonstrate an absolute requirement for Garz during Drosophila development.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.4161/cl.1.2.15512DOIArticle
http://www.tandfonline.com/doi/abs/10.4161/cl.1.2.15512PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3116586/PubMed CentralArticle
ORCID:
AuthorORCID
Zinn, Kai0000-0002-6706-5605
Additional Information:© 2011 Landes Bioscience. Submitted: 02/08/11; Revised: 03/11/11; Accepted: 03/17/11. We thank Dr. Roland Le Borgne and the late Dr. John Sisson for antibodies. This work was supported by scholarships from NSERC and OGS to J.B., grants from NSERC (#262166) and The Cancer Research Society, Inc., to J.A.B., a National Science Foundation award (NSF0744471) to E.S. and National Institutes of Health grant (NS43416.) to K.Z.
Funders:
Funding AgencyGrant Number
Natural Sciences and Engineering Research Council of Canada (NSERC)262166
Ontario Graduate Scholarship (OGS)UNSPECIFIED
Cancer Research SocietyUNSPECIFIED
NSFNSF0744471
NIHNS43416
Subject Keywords:Arf, Sec7 domain, Garz, GBF1, membrane traffic, Drosophila
Issue or Number:2
PubMed Central ID:PMC3116586
Record Number:CaltechAUTHORS:20151218-090549591
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20151218-090549591
Official Citation:Tomasz Szul, Jason Burgess, Mili Jeon, Guillermo Marques, Julie A. Brill & Elizabeth Sztul (2011) The Garz Sec7 domain guanine nucleotide exchange factor for Arf regulates salivary gland development in Drosophila, Cellular Logistics, 1:2, 69-76, DOI: 10.4161/cl.1.2.15512
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63059
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:18 Dec 2015 23:55
Last Modified:03 Oct 2019 09:24

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