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Microbial eukaryotic distributions and diversity patterns in a deep-sea methane seep ecosystem

Pasulka, Alexis L. and Levin, Lisa A. and Steele, Josh A. and Case, David H. and Landry, Michael R. and Orphan, Victoria J. (2016) Microbial eukaryotic distributions and diversity patterns in a deep-sea methane seep ecosystem. Environmental Microbiology, 18 (9). pp. 3022-3043. ISSN 1462-2912. https://resolver.caltech.edu/CaltechAUTHORS:20151221-150217687

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Abstract

Although chemosynthetic ecosystems are known to support diverse assemblages of microorganisms, the ecological and environmental factors that structure microbial eukaryotes (heterotrophic protists and fungi) are poorly characterized. In this study, we examined the geographic, geochemical and ecological factors that influence microbial eukaryotic composition and distribution patterns within Hydrate Ridge, a methane seep ecosystem off the coast of Oregon using a combination of high-throughput 18S rRNA tag sequencing, terminal restriction fragment length polymorphism fingerprinting, and cloning and sequencing of full-length 18S rRNA genes. Microbial eukaryotic composition and diversity varied as a function of substrate (carbonate versus sediment), activity (low activity versus active seep sites), sulfide concentration, and region (North versus South Hydrate Ridge). Sulfide concentration was correlated with changes in microbial eukaryotic composition and richness. This work also revealed the influence of oxygen content in the overlying water column and water depth on microbial eukaryotic composition and diversity, and identified distinct patterns from those previously observed for bacteria, archaea and macrofauna in methane seep ecosystems. Characterizing the structure of microbial eukaryotic communities in response to environmental variability is a key step towards understanding if and how microbial eukaryotes influence seep ecosystem structure and function.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1111/1462-2920.13185DOIArticle
http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.13185/abstractPublisherArticle
ORCID:
AuthorORCID
Levin, Lisa A.0000-0002-2858-8622
Orphan, Victoria J.0000-0002-5374-6178
Additional Information:© 2016 Society for Applied Microbiology and John Wiley & Sons Ltd. Received 7 October, 2015; revised 3 December, 2015; accepted 8 December, 2015. First published: 25 January 2016. Special Issue on Ecophysiology of Anaerobes and their Habitat. We are grateful to the captain and crew of the R/V Atlantis and the JASON pilots who helped make sampling possible. We offer thanks to J. Marlow, A. Dekas, A. Green-Saxena, S. Connon and many others for help at sea and/or in the laboratory. In particular, we would like to thank R. Leon for help developing at-sea methods, J. Glass for providing sulfide data, G. Chadwick, E. Wilbanks and C. Skennerton for programming assistance, G. Mendoza for helpful input regarding the statistical analyses, and E. Allen for insightful discussions about sample processing and analyses. Support for this research was provided by grant OCE 0825791, OCE 0826254 and OCE 0939557 from the US National Science Foundation (NSF). VJO is supported by a grant from the Gordon and Betty Moore Foundation Marine Microbial Initiative (GBMF3780). NSF Graduate Research Fellowships supported ALP and DHC, and a P.E.O Scholar Award additionally supported ALP.
Funders:
Funding AgencyGrant Number
NSFOCE-0825791
NSFOCE-0826254
NSFOCE-0939557
Gordon and Betty Moore FoundationGBMF3780
NSF Graduate Research FellowshipUNSPECIFIED
P.E.O. InternationalUNSPECIFIED
Issue or Number:9
Record Number:CaltechAUTHORS:20151221-150217687
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20151221-150217687
Official Citation:Pasulka, A. L., Levin, L. A., Steele, J. A., Case, D. H., Landry, M. R. and Orphan, V. J. (2016), Microbial eukaryotic distributions and diversity patterns in a deep-sea methane seep ecosystem. Environ Microbiol, 18: 3022–3043. doi:10.1111/1462-2920.13185
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63109
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:22 Dec 2015 19:15
Last Modified:09 Mar 2020 13:19

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