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The sea urchin profilin gene is specifically expressed in mesenchyme cells during gastrulation

Smith, L. Courtney and Harrington, Michael G. and Britten, Roy J. and Davidson, Eric H. (1994) The sea urchin profilin gene is specifically expressed in mesenchyme cells during gastrulation. Developmental Biology, 164 (2). pp. 463-474. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20151223-092605241

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Abstract

Eggs and embryos of the purple sea urchin (Strongylocentrotus purpuratus) contain profilin that is partly supplied from maternal sources and partly produced by the gastrula. The maternal profilin protein content is about 13 μM and it persists in the embryo at least through gastrulation. Transcript quantitation from probe excess titrations show that very few profilin gene transcripts are present in the embryo during cleavage, but that they increase at the onset of gastrulation. By in situ hybridization, the newly synthesized profilin transcripts are localized in mesenchyme cells. Profilin gene expression increases when mesenchyme cells initiate migration and filopodial extension and retraction. We show that there are three isoforms of maternal profilin protein produced from the single copy gene during oogenesis. However, the blastula stage embryo only produces the major isoform, whereas the acidic isoform is produced in the early stages of gastrulation and the basic isoform appears by the end of gastrulation. Based on transcript prevalence and protein production rates, our calculations indicate that the amount of new protein produced in the mesenchyme cells in 12 hr is at maximum <2% of that supplied from maternal sources. Because of the large amount of maternally supplied profilin present in the egg and embryo, we suggest that it may be used in the cytokinetic processes of cleavage. Alternatively, because of the small amount of embryonically produced profilin, we suggest that it may function in the cytoskeletal shape changes required for filopodial extension and motility in the mesenchyme cells during gastrulation.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1006/dbio.1994.1216DOIArticle
http://www.sciencedirect.com/science/article/pii/S0012160684712164PublisherArticle
ORCID:
AuthorORCID
Harrington, Michael G.0000-0002-7923-8032
Additional Information:© 1994 by Academic Press, Inc. Accepted April 29, 1994. This research was supported by a grant from the NIH (HD-05753) to E.H.D. and by grants from the NSF (MCB-9219330) to L.C.S. and (STCDIR-8809719) to M.G.H.
Funders:
Funding AgencyGrant Number
NIHHD-05753
NSFMCB-9219330
NSFSTCDIR-8809719
Issue or Number:2
Record Number:CaltechAUTHORS:20151223-092605241
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20151223-092605241
Official Citation:L. Courtney Smith, Michael G Harrington, Roy J. Britten, Eric H. Davidson, The sea urchin profilin gene is specifically expressed in mesenchyme cells during gastrulation, Developmental Biology, Volume 164, Issue 2, 1994, Pages 463-474, ISSN 0012-1606, http://dx.doi.org/10.1006/dbio.1994.1216. (http://www.sciencedirect.com/science/article/pii/S0012160684712164)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63170
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:23 Dec 2015 18:09
Last Modified:03 Oct 2019 09:25

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