Calzone, F. J. and Grainger, J. and Coffman, J. A. and Davidson, E. H. (1997) Extensive maternal representation of DNA-binding proteins that interact with regulatory target sites of the Strongylocentrotus purpuratus CyIIIa gene. Molecular Marine Biology and Biotechnology, 6 (2). pp. 79-83. ISSN 1053-6426. https://resolver.caltech.edu/CaltechAUTHORS:20151223-160210513
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Abstract
Nine different embryonic transcription factors interact at specific target sites in the cis-regulatory domain of the Strongylocentrotus purpuratus CyIIIa gene. We tested eight of these site sequences and show here that for every one a DNA-binding protein was present in unfertilized egg cytoplasm. The concentrations of active DNA-binding proteins per egg were estimated. We also present a new and convenient method for preparation of a material cytoplasmic fraction that retains these factors in active form.
Item Type: | Article |
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Additional Information: | © 1997 Springer. |
Issue or Number: | 2 |
Record Number: | CaltechAUTHORS:20151223-160210513 |
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20151223-160210513 |
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. |
ID Code: | 63204 |
Collection: | CaltechAUTHORS |
Deposited By: | George Porter |
Deposited On: | 24 Feb 2016 22:45 |
Last Modified: | 03 Oct 2019 09:26 |
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