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Extensive maternal representation of DNA-binding proteins that interact with regulatory target sites of the Strongylocentrotus purpuratus CyIIIa gene

Calzone, F. J. and Grainger, J. and Coffman, J. A. and Davidson, E. H. (1997) Extensive maternal representation of DNA-binding proteins that interact with regulatory target sites of the Strongylocentrotus purpuratus CyIIIa gene. Molecular Marine Biology and Biotechnology, 6 (2). pp. 79-83. ISSN 1053-6426. https://resolver.caltech.edu/CaltechAUTHORS:20151223-160210513

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Abstract

Nine different embryonic transcription factors interact at specific target sites in the cis-regulatory domain of the Strongylocentrotus purpuratus CyIIIa gene. We tested eight of these site sequences and show here that for every one a DNA-binding protein was present in unfertilized egg cytoplasm. The concentrations of active DNA-binding proteins per egg were estimated. We also present a new and convenient method for preparation of a material cytoplasmic fraction that retains these factors in active form.


Item Type:Article
Additional Information:© 1997 Springer.
Issue or Number:2
Record Number:CaltechAUTHORS:20151223-160210513
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20151223-160210513
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63204
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:24 Feb 2016 22:45
Last Modified:03 Oct 2019 09:26

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