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Comparison of two techniques for targeting the production of monoclonal antibodies against particular antigens

Ou, Susan Ker-hwa and McDonald, Colin and Patterson, Paul H. (1991) Comparison of two techniques for targeting the production of monoclonal antibodies against particular antigens. Journal of Immunological Methods, 145 (1-2). pp. 111-118. ISSN 0022-1759. https://resolver.caltech.edu/CaltechAUTHORS:20151229-160905165

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Abstract

We have compared the efficacy of two methods for enhancing the probability of producing monoclonal antibodies against particular target antigens in a complex mixture. These methods use two tissue extracts, one extract that contains (A) and one extract that does not contain (B) the target antigen(s) of interest. In the chemical immunosuppression approach, cyclophosphamide is used to suppress the mouse's response to common antigens in extract B before injection of extract A containing the target antigens. In the tolerization approach, neonatal mice are tolerized against the common antigens in extract B before injection of extract A containing the target antigens. Although small numbers of animals were used in this initial comparison, immunodot assays clearly indicate that the cyclophosphamide immunosuppression method yields significantly more monoclonal antibodies specific for the target antigen-containing extract A than does the tolerization method.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0022-1759(91)90316-8DOIArticle
http://www.sciencedirect.com/science/article/pii/0022175991903168PublisherArticle
Additional Information:© 1991 Elsevier. (Received 30 April 1991; revised received 22 July 1991; accepted 30 July 1991) We would like to thank Dr. John Allman for providing us the monkey brain and Zaven Kaprielian, Ming-Ji Fann and James Ou for helpful suggestions. This work was uspported by NSF grant DIR-8712123.
Funders:
Funding AgencyGrant Number
NSFDIR-8712123
Subject Keywords:Immunosuppression; Cyclophosphamide; Neonatal tolerization
Issue or Number:1-2
Record Number:CaltechAUTHORS:20151229-160905165
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20151229-160905165
Official Citation:Susan Ker-hwa Ou, Colin McDonald, Paul H. Patterson, Comparison of two techniques for targeting the production of monoclonal antibodies against particular antigens, Journal of Immunological Methods, Volume 145, Issues 1–2, 15 December 1991, Pages 111-118, ISSN 0022-1759, http://dx.doi.org/10.1016/0022-1759(91)90316-8. (http://www.sciencedirect.com/science/article/pii/0022175991903168)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63254
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:10 Feb 2016 22:39
Last Modified:03 Oct 2019 09:26

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