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Further studies of the distribution of CDF/LIF mRNA

Patterson, Paul H. and Fann, Ming-Ji (1992) Further studies of the distribution of CDF/LIF mRNA. In: Polyfunctional Cytokines : IL-6 and LIF. Ciba Foundation Symposium. No.167. John Wiley & Sons , Chichester, pp. 125-140. ISBN 9780470514269.

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Differentiation choices in the haemopoietic and nervous systems are controlled in part by instructive factors. The cholinergic differentiation factor (CDF, also known as leukaemia inhibitory factor, LIF) affects the development of cultured cells from both systems. To understand the role of CDF/LIF during normal development in vivo, we have begun to localize its mRNA in the late fetal and postnatal rat. Application of reverse transcriptase-polymerase chain reaction and RNase protection methods reveals that CDF/LIF mRNA levels are developmentally modulated in both haemopoietic and neural tissues. A target tissue of cholinergic sympathetic neurons, the footpads that contain the sweat glands, express high levels of this mRNA (relative to mRNA for actin and β2-microglobulin). Levels in targets of noradrenergic neurons are lower, but do undergo significant changes during development. Signals are also detected in selective regions of the adult brain, and in embryonic skeletal muscle. This finding in muscle may be significant for motor neurons, because CDF/LIF is a trophic factor for these neurons in culture. Embryonic liver, neonatal thymus and postnatal spleen express CDF/LIF mRNA, and expression in gut is the highest of all tissues examined. The selective tissue distribution and developmental modulation of CDF/LIF mRNA expression support a role for this factor in the normal development of several organ systems.

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Additional Information: © Ciba Foundation 1992. We thank David Anderson for use of his equipment and Derek Stemple and Susan Birren for advice and reagents. Tetsuo Yamamori initiated the studies of postnatal tissues and Xiao-Yi Xie participated in the beginning of the studies of embryonic tissues. This work was supported by a Jacob Javits Investigator Award from the National Institute of Neurological Disorders and Stroke to P.H.P., and by fellowships to M.-J.F. from the Helen G. and Arthur McCallum Foundation, the Ministry of Education, Taiwan, R.O.C., and a Li Ming Scholarship.
Funding AgencyGrant Number
National Institute of Neurological Disorders and Stroke (NINDS)UNSPECIFIED
Helen G. and Arthur McCallum FoundationUNSPECIFIED
Ministry of Education (Taipei)UNSPECIFIED
Li Ming ScholarshipUNSPECIFIED
Subject Keywords:differentiation; cholinergic differentiation factor; RNAse protection; embryonic liver; polyfunctional cytokines
Series Name:Ciba Foundation Symposium
Issue or Number:167
Record Number:CaltechAUTHORS:20151229-161008380
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Official Citation:Patterson, P. H. and Fann, M.-J. (2007) Further Studies of the Distribution of CDF/LIF mRNA, in Ciba Foundation Symposium 167 - Polyfunctional Cytokines: IL-6 and LIF (eds G. R. Bock, J. Marsh and K. Widdows), John Wiley & Sons, Ltd., Chichester, UK. doi: 10.1002/9780470514269.ch8
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63255
Deposited By: George Porter
Deposited On:10 Feb 2016 21:24
Last Modified:10 Nov 2021 23:14

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