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A more efficient and economical approach for monoclonal antibody production

Ou, Susan Ker-hwa and Patterson, Paul H. (1997) A more efficient and economical approach for monoclonal antibody production. Journal of Immunological Methods, 209 (1). pp. 105-108. ISSN 0022-1759. https://resolver.caltech.edu/CaltechAUTHORS:20151229-231808369

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Abstract

We describe here an efficient, economical way to produce monoclonal antibodies in tissue culture flasks. Hybridoma cells are cultured in horizontal tissue culture flasks until 90% confluent, and then the flasks are filled with culture medium and placed in a standing position and left in the incubator for 3–4 weeks until most of the cells have died. At this time, the culture fluid is harvested for antibodies. With this method, which involves only a single cell plating and one change of medium, the yield of antibodies from one tissue culture flask is 6- to 9-fold higher than is obtained by the usual method of sequential replating and harvesting of cells and media. This approach therefore saves labor as well as costly materials, and minimizes risk of contamination.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0022-1759(97)00156-7DOIArticle
http://www.sciencedirect.com/science/article/pii/S0022175997001567PublisherArticle
Additional Information:© 1997 Elsevier. Received 1 July 1997; revised 5 September 1997; accepted 5 September 1997.
Subject Keywords:Antibody production; Antibody yield
Issue or Number:1
Record Number:CaltechAUTHORS:20151229-231808369
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20151229-231808369
Official Citation:Susan Ker-hwa Ou, Paul H Patterson, A more efficient and economical approach for monoclonal antibody production, Journal of Immunological Methods, Volume 209, Issue 1, 10 November 1997, Pages 105-108, ISSN 0022-1759, http://dx.doi.org/10.1016/S0022-1759(97)00156-7. (http://www.sciencedirect.com/science/article/pii/S0022175997001567)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63261
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:10 Feb 2016 22:48
Last Modified:03 Oct 2019 09:26

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