Ou, Susan Ker-hwa and Patterson, Paul H. (1997) A more efficient and economical approach for monoclonal antibody production. Journal of Immunological Methods, 209 (1). pp. 105-108. ISSN 0022-1759. doi:10.1016/S0022-1759(97)00156-7. https://resolver.caltech.edu/CaltechAUTHORS:20151229-231808369
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Abstract
We describe here an efficient, economical way to produce monoclonal antibodies in tissue culture flasks. Hybridoma cells are cultured in horizontal tissue culture flasks until 90% confluent, and then the flasks are filled with culture medium and placed in a standing position and left in the incubator for 3–4 weeks until most of the cells have died. At this time, the culture fluid is harvested for antibodies. With this method, which involves only a single cell plating and one change of medium, the yield of antibodies from one tissue culture flask is 6- to 9-fold higher than is obtained by the usual method of sequential replating and harvesting of cells and media. This approach therefore saves labor as well as costly materials, and minimizes risk of contamination.
| Item Type: | Article | |||||||||
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| Additional Information: | © 1997 Elsevier. Received 1 July 1997; revised 5 September 1997; accepted 5 September 1997. | |||||||||
| Subject Keywords: | Antibody production; Antibody yield | |||||||||
| Issue or Number: | 1 | |||||||||
| DOI: | 10.1016/S0022-1759(97)00156-7 | |||||||||
| Record Number: | CaltechAUTHORS:20151229-231808369 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20151229-231808369 | |||||||||
| Official Citation: | Susan Ker-hwa Ou, Paul H Patterson, A more efficient and economical approach for monoclonal antibody production, Journal of Immunological Methods, Volume 209, Issue 1, 10 November 1997, Pages 105-108, ISSN 0022-1759, http://dx.doi.org/10.1016/S0022-1759(97)00156-7. (http://www.sciencedirect.com/science/article/pii/S0022175997001567) | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 63261 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | George Porter | |||||||||
| Deposited On: | 10 Feb 2016 22:48 | |||||||||
| Last Modified: | 10 Nov 2021 23:14 |
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