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Confocal Imaging of Living Cells in Intact Embryos

Kulesa, Paul M. and Fraser, Scott E. (1999) Confocal Imaging of Living Cells in Intact Embryos. In: Confocal Microscopy Methods and Protocols. Methods in Molecular Biology. No.122. Humana Press , Totowa, NJ, pp. 205-222. ISBN 978-0-89603-526-3.

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How individual cells and tissues organize and coordinate to form the structure of a developing embryo is a major question in developmental biology. Equally intriguing is how cells in adult tissue respond during wound healing and tissue regeneration. In these morphogenetic processes there can be a wide variety of cell behaviors: individual cells may change shape or divide, migrate to different areas, or differentiate to form a pattern. Entire tissue layers can spread across a surface, fold up or cavitate to form a three-dimensional structure. For example, during formation of the neural tube in chick embryos, the neural epithelium folds up from a flat sheet of cells to form a tube with a hollow lumen. As the neural tube becomes shaped into various regions of the nervous system, the neural crest cells emerge from the dorsal midline of the neural tube and migrate to pattern the peripheral nervous system. A main theme in these examples is that the cell and tissue events are dynamic, on a time scale from minutes to days, covering spatial regions ranging in size from microns to millimeters.

Item Type:Book Section
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Fraser, Scott E.0000-0002-5377-0223
Additional Information:© 1999 Humana Press.
Series Name:Methods in Molecular Biology
Issue or Number:122
Record Number:CaltechAUTHORS:20151231-003932619
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63282
Deposited By: George Porter
Deposited On:08 Feb 2016 22:22
Last Modified:10 Nov 2021 23:14

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