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Dynamic In Vivo Imaging of Mammalian Hematovascular Development Using Whole Embryo Culture

Jones, Elizabeth A. V. and Baron, Margaret H. and Fraser, Scott E. and Dickinson, Mary E. (2005) Dynamic In Vivo Imaging of Mammalian Hematovascular Development Using Whole Embryo Culture. In: Developmental Hematopoiesis: Methods and Protocols. Methods in Molecular Medicine. Vol.III. No.105. Humana Press , Totowa, NJ, pp. 381-394. ISBN 978-1-58829-296-4.

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The yolk sac is the initial site of hematopoiesis in the mammalian embryo. As the embryo develops, blood vessels form around primitive erythroblasts to connect the yolk sac to the embryo, delivering newly formed blood cells to the embryonic circulation. The limited accessibility of the mammalian embryo has made it difficult to study the dynamic changes in cellular development during the formation of the early hematovascular system. Therefore, we have developed a culture system for studying early hematopiesis, vasculogenesis. and angiogenesis in the mouse embryo. Early embryos (E7.5–E9.5) can be grown on the microscope stage to study the dynamics as vessels form and circulation begins. In addition, this mouse embryo culture system provides an excellent model for understanding the interplay between flow dynamics and cellular development.

Item Type:Book Section
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Fraser, Scott E.0000-0002-5377-0223
Additional Information:© 2005 Humana Press.
Subject Keywords:Post-implantation embryo; embryo cultur;e microscopy; time-lapse imaging; yolk sac; primitive hematopoiesis; vasculogenesis
Series Name:Methods in Molecular Medicine
Issue or Number:105
Record Number:CaltechAUTHORS:20151231-155447872
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63283
Deposited By: George Porter
Deposited On:02 Feb 2016 21:29
Last Modified:10 Nov 2021 23:14

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