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Clonal analysis of the avian neural crest: Migration and maturation of mixed neural crest clones injected into host chicken embryos

Bronner-Fraser, Marianne and Sieber-Blum, Maya and Cohen, Alan M. (1980) Clonal analysis of the avian neural crest: Migration and maturation of mixed neural crest clones injected into host chicken embryos. Journal of Comparative Neurology, 193 (2). pp. 423-434. ISSN 0021-9967. doi:10.1002/cne.901930209. https://resolver.caltech.edu/CaltechAUTHORS:20160101-142438234

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Abstract

Quail neural crest cells were grown in vitro at clonal density for 7 or 10 days. Mixed neural crest colonies and clones (containing both pigmented and unpigmented cells) were implanted into the trunk region of 2 1/2-day-old host chicken embryos by a previously described injection technique (Bronner and Cohen, '79). Here we describe the migratory behavior and subsequent phenotypic expression of the injected cells. Unpigmented cells and pigmented cells both migrated along the ventral neural crest pathway; there were, however, some differences in migratory behavior between the two cell types. After 3 days in vivo, unpigmented quail neural crest cells contributed to the sympathetic ganglion, adrenal medulla, and/or aortic plexus in the host. Many of the unpigmented cells became catecholamine-containing neuroblasts. Unpigmented cells were never observed in the gonads or the gut, but localized only in regions normally populated by trunk neural crest precursors to neurons and supportive cells. Melanocytes derived from the same precursor, however, were often found in the gonads or gut, in addition to normal neural crest locations in the trunk. These results demonstrate that quail neural crest cells grown in tissue culture for 7 days or more retain the ability to migrate and contribute to normal neural crest structures when placed in the embryonic environment. Under the conditions described, a single neural crest cell gave rise to daughter cells expressing the melanotic phenotype (detected in tissue culture) and adrenergic phenotypes (detected after injection in vivo). This demonstrates that at least some single cells of the premigratory crest in the trunk region are pluripotent.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/cne.901930209DOIArticle
http://onlinelibrary.wiley.com/doi/10.1002/cne.901930209/abstractPublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 1980 Alan R. Liss, Inc. This research was supported by U.S. Public Health Service grant HD-07389 and a Basic Research Grant from the National Foundation - March of Dimes awarded to A.M.C., by NIH Research Service Award HD05646 to M.S.-B., and by a U.S. Public Health Service Predoctoral Training Grant 5T32GM07231 to M.B.-F.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)HD-07389
National Foundation - March of DimesUNSPECIFIED
U.S. Public Health Service (USPHS)HD-05646
NIH Predoctoral Fellowship5T32GM07231
Issue or Number:2
DOI:10.1002/cne.901930209
Record Number:CaltechAUTHORS:20160101-142438234
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160101-142438234
Official Citation:Bronner-Fraser, M., Sieber-blum, M. and Cohen, A. M. (1980), Clonal analysis of the avian neural crest: Migration and maturation of mixed neural crest clones injected into host chicken embryos. J. Comp. Neurol., 193: 423–434. doi: 10.1002/cne.901930209
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63286
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:04 Feb 2016 21:22
Last Modified:10 Nov 2021 23:14

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