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Distribution and function of tenascin during cranial neural crest development in the chick

Bronner-Fraser, M. (1988) Distribution and function of tenascin during cranial neural crest development in the chick. Journal of Neuroscience Research, 21 (2-4). pp. 135-147. ISSN 0360-4012. http://resolver.caltech.edu/CaltechAUTHORS:20160101-144022515

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Abstract

Tenascin is a glycoprotein associated with the extra-cellular matrix and the surface of some cell types. Here, the distribution and possible function of tenascin have been examined along the pathways followed by cranial neural crest cells. During early stages of neural crest migration, tenascin was observed in a dense matrix surrounding premigratory cranial neural crest cells. Along the neural tube, tenascin immunoreactivity was observed in a dorsoventral gradient and was also noted under the ectoderm and around the notochord. During advanced neural crest migration, tenascin immunoreactivity colocalized with and appeared to be on the surface of migrating neural crest cells. At later stages, tenascin was present around the otic vesicles, retina, lens, and in an interstitial matrix in the region of the branchial arches. At the level of the occipital somites, tenascin immunoreactivity was observed around the neural tube, notochord, dermamyotome, and on the basal surface of the ectoderm. Tenascin was also observed in an interstitial matrix within the sclerotome. At early stages of vagal neural crest migration, immunoreactivity was uniform within the sclerotome, whereas at later stages tenascin colocalized with vagal neural crest cells within the rostral half of each sclerotome. The possible function of tenascin was tested by injecting antitenascin antibodies lateral to the mesencephalic neural tube. Two predominant defects were noted in injected embryos: (1) ectopic aggregates of cranial neural crest cells external to the neural tube and sometimes located on the apical side of the ectoderm; and (2) open and deformed neural tubes. Both the distribution and results of the perturbation experiment suggest that tenascin is required for proper cranial neural crest migration.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/jnr.490210206DOIArticle
http://onlinelibrary.wiley.com/doi/10.1002/jnr.490210206/abstractPublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, M.0000-0003-4274-1862
Additional Information:© 1988 Alan R. Liss, Inc. Special Issue: Neuroembryology -- Cellular and Molecular Approaches I thank Drs. Roberto Perris, Thomas Lallier, and Danuta Krotoski for helpful comments on the manuscript and Geogia Guillory, Mary Flowers, and Michael Artinger for excellent technical assistance. This work was supported by USPHS grant HD-15527 and by Basic Research Grant 1-896 from the March of Dimes. The author is a Sloan Foundation Research Fellow.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)HD-15527
March of Dimes1-896
Alfred P. Sloan FoundationUNSPECIFIED
Subject Keywords:neural crest migration; immunoreactivity; neural tube defects; myotendinous antigen
Record Number:CaltechAUTHORS:20160101-144022515
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160101-144022515
Official Citation:Bronner-Fraser, M. (1988), Distribution and function of tenascin during cranial neural crest development in the chick. J. Neurosci. Res., 21: 135–147. doi: 10.1002/jnr.490210206
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63290
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:04 Feb 2016 23:12
Last Modified:04 Feb 2016 23:12

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