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Distribution of integrins and their ligands in the trunk of Xenopus laevis during neural crest cell migration

Krotoski, Danuta and Bronner-Fraser, Marianne (1990) Distribution of integrins and their ligands in the trunk of Xenopus laevis during neural crest cell migration. Journal of Experimental Zoology, 253 (2). pp. 139-150. ISSN 0022-104X. https://resolver.caltech.edu/CaltechAUTHORS:20160101-174449164

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Abstract

We have examined the distribution in Xenopus embryos of β1 subunits of integrin, as recognized by cross-reactive antibodies against the avian integrin beta1 subunit. These antibodies recognize a doublet of bands of approximately 120 kD in Xenopus embryos. The distribution pattern of these integrin cell surface receptors was compared with that of two possible ligands, fibronectin and laminin, in the extracellular matrix during the time of neural crest cell migration. Integrin immunoreactivity in the early neurula was observed lightly outlining somite and epidermal cells and the notochord. The integrin immunostaining increased with developmental age and was observed on most cell types in the embryo but was particularly notable in the intersomitic clefts through which motoraxons grow. The immunoreactivity in this region was not, however, wholly on the axon surfaces, since intersomitic integrin remained detectable in embryos in which the neural tube had been ablated. Fibronectin and laminin were more extensively distributed than integrin at all stages examined. Immunoreactivity for both was observed around the neural tube, notochord, somites, epidermis, dorsal mesentery, and lateral plate mesoderm. The distribution of laminin and fibronectin around the somites was particularly interesting since it was non-uniform and similar to that of integrin. Strongest staining was observed in the intersomitic clefts, and weakest staining was observed on the medial surface of the somites, which faces the neural tube and notochord. The major differences in distribution pattern between the fibronectin and laminin immunoreactivities were that only fibronectin was detected in the mesenchyme of the dorsal fin. Our results demonstrate that a molecule homologous to avian integrin is present in Xenopus embryos during neural crest cell migration and motoraxon outgrowth. Its presence in the intersomitic clefts and on the surface of many embryonic cell types together with the abundant distribution of its ligands are consistent with a potentially important developmental function in neurite outgrowth and/or muscle development.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/jez.1402530204DOIArticle
http://onlinelibrary.wiley.com/doi/10.1002/jez.1402530204/abstractPublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 1990 Wiley-Liss. Received April 4, 1989; accepted July 20, 1989. We thank Drs. Roberto Perris and Scott Fraser for critical reading of the manuscript, Michael Artinger for technical assistance, and Thomas Lallier for help with immunoblots. This work was supported by USPHS grant HD15527 and NSF grant BNS 8607760.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)HD-15527
NSFBNS 8607760
Issue or Number:2
Record Number:CaltechAUTHORS:20160101-174449164
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160101-174449164
Official Citation:Krotoski, D. and Bronner-Fraser, M. (1990), Distribution of integrins and their ligands in the trunk of Xenopus laevis during neural crest cell migration. J. Exp. Zool., 253: 139–150. doi: 10.1002/jez.1402530204
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63291
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:27 Jan 2016 19:23
Last Modified:03 Oct 2019 09:27

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