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Japanese Quail: An Efficient Animal Model for the Production of Transgenic Avians

Poynter, Greg and Huss, David and Lansford, Rusty (2009) Japanese Quail: An Efficient Animal Model for the Production of Transgenic Avians. Cold Spring Harbor Protocols, 2009 (1). Art. No. pdb.emo112. ISSN 1940-3402. https://resolver.caltech.edu/CaltechAUTHORS:20160107-121845140

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Abstract

The ability to generate transgenic mice has been a powerful tool in studying functional genomics, and much of our knowledge about developmental biology has come from the study of chicken embryology. Unfortunately, the availability of molecular genetic techniques, such as transgenics and knockouts, has been limited for developmental biologists using avian animal models. Efforts to develop a system for the rapid production of transgenic chickens have met with many obstacles, including high animal husbandry costs and long generational times. Recently, the Japanese quail has proven to be an excellent model organism for the production of transgenic avians using lentiviral vectors. The relatively small size of the adults, short time to sexual maturity, and prodigious egg production of the Japanese quail make development of transgenic lines less labor- and space-intensive compared to chickens. The high degree of homology between chicken and quail genomes allows researchers to design highly specific DNA constructs for the production of transgenic birds. In addition, transgenic quail offer all of the advantages of the classic avian developmental model system, such as the ability to readily produce quail:chick transplant chimeras. Finally, Japanese quail are ideal for in ovo imaging of embryos expressing fluorescent reporters introduced from a transgene and/or electroporation. Here, we provide detailed methods for generating transgenic quail using high-titer lentivirus.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1101/pdb.emo112DOIArticle
http://cshprotocols.cshlp.org/content/2009/1/pdb.emo112.abstractPublisherArticle
http://resolver.caltech.edu/CaltechAUTHORS:20160107-121441963Related ItemBook Chapter
ORCID:
AuthorORCID
Lansford, Rusty0000-0002-2159-3699
Additional Information:© 2009 Cold Spring Harbor Laboratory Press.
Issue or Number:1
Record Number:CaltechAUTHORS:20160107-121845140
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160107-121845140
Official Citation:Japanese Quail: An Efficient Animal Model for the Production of Transgenic Avians Greg Poynter, David Huss, and Rusty Lansford Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.emo112
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63453
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:08 Jan 2016 16:06
Last Modified:03 Oct 2019 09:28

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