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Characterization of Antibody Bipolar Bridging Mediated by the Human Cytomegalovirus Fc receptor gp68

Ndjamen, Blaise and Joshi, Devashish S. and Fraser, Scott E. and Bjorkman, Pamela J. (2016) Characterization of Antibody Bipolar Bridging Mediated by the Human Cytomegalovirus Fc receptor gp68. Journal of Virology, 90 (6). pp. 3262-3567. ISSN 0022-538X. PMCID PMC4810659. doi:10.1128/JVI.02855-15. https://resolver.caltech.edu/CaltechAUTHORS:20160111-100543517

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Abstract

The human cytomegalovirus glycoprotein gp68 functions as an Fc receptor for host IgGs and can form antibody bipolar bridging (ABB) complexes in which gp68 binds the Fc region of an antigen-bound IgG. Here we show that gp68-mediated endocytosis transports ABB complexes into endosomes, after which the complex is routed to lysosomes, presumably for degradation. These results suggest gp68 contributes to evasion of IgG-mediated immune responses by mediating destruction of host IgG and viral antigens.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1128/JVI.02855-15DOIArticle
http://jvi.asm.org/content/90/6/3262.longPublisherArticle
http://jvi.asm.org/content/90/6/3262/suppl/DCSupplementalPublisherSupplementary Material
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4810659/PubMed CentralArticle
ORCID:
AuthorORCID
Fraser, Scott E.0000-0002-5377-0223
Bjorkman, Pamela J.0000-0002-2277-3990
Additional Information:© 2016 American Society for Microbiology. Received 11 November 2015 Accepted 30 December 2015. Accepted manuscript posted online 6 January 2016. Editor: K. Frueh. We thank Homayon Ghiasi for the gD gene, Hartmut Hengel and Elizabeth R. Sprague for the gp68 gene, Alex Farley for the anti-gD gene constructs, the Caltech Protein Expression Center for expression of antibodies used for internalization, Yunji Wu for purified 2G12 IgG, Marta Murphy for help making figures, and members of the Bjorkman lab for critical reading of the manuscript. This work was supported by the National Institutes of Health (grant R01 AI041239 to P.J.B.) and the Irvington Institute Fellowship Program of the Cancer Research Institute (postdoctoral fellowship to B.N.; www.cancerresearch.org/grants-programs/grants-fellowships). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. FUNDING INFORMATION: HHS | NIH | National Institute of Allergy and Infectious Diseases (NIAID) http://dx.doi.org/10.13039/100000060R01 AI041239 Pamela J. Bjorkman Cancer Research Institute (CRI) http://dx.doi.org/10.13039/100000884 Blaise Ndjamen
Funders:
Funding AgencyGrant Number
NIHR01 AI041239
Cancer Research InstituteUNSPECIFIED
Issue or Number:6
PubMed Central ID:PMC4810659
DOI:10.1128/JVI.02855-15
Record Number:CaltechAUTHORS:20160111-100543517
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160111-100543517
Official Citation:Characterization of Antibody Bipolar Bridging Mediated by the Human Cytomegalovirus Fc Receptor gp68 Blaise Ndjamen, Devashish S. Joshi, Scott E. Fraser, and Pamela J. Bjorkman J. Virol. March 2016 90:6 3262-3267; Accepted manuscript posted online 6 January 2016, doi:10.1128/JVI.02855-15
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63537
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:13 Jan 2016 00:23
Last Modified:10 Nov 2021 23:17

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