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Sunlight-Activated Propidium Monoazide Pretreatment for Differentiation of Viable and Dead Bacteria by Quantitative Real-Time Polymerase Chain Reaction

Xie, Xing and Wang, Siwen and Jiang, Sunny C. and Bahnemann, Janina and Hoffmann, Michael R. (2016) Sunlight-Activated Propidium Monoazide Pretreatment for Differentiation of Viable and Dead Bacteria by Quantitative Real-Time Polymerase Chain Reaction. Environmental Science and Technology Letters, 3 (2). pp. 57-61. ISSN 2328-8930. https://resolver.caltech.edu/CaltechAUTHORS:20160111-103924491

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Abstract

Polymerase chain reaction (PCR)-based methods have been developed and increasingly used for rapid and sensitive detection of pathogens in water samples to better protect public health. A propidium monoazide (PMA) pretreatment can help to differentiate between viable and dead cells, but the photoactivation of PMA normally requires the use of an energy-consuming halogen light, which is not suitable for off-the-grid applications. Herein, we investigate sunlight as an alternative light source. Our results suggest that sunlight can successfully activate PMA, and the sunlight-activated PMA pretreatment can effectively reduce the amplification of DNA derived from dead cells in PCR assays. Potentially, a sunlight-activated PMA pretreatment unit can be integrated into a lab-on-a-chip PCR device for off-the-grid microbial detection and quantification.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/acs.estlett.5b00348DOIArticle
http://pubs.acs.org/doi/10.1021/acs.estlett.5b00348PublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/acs.estlett.5b00348PublisherSupporting Information
ORCID:
AuthorORCID
Hoffmann, Michael R.0000-0002-0432-6564
Additional Information:© 2016 American Chemical Society. Received: December 15, 2015; Revised: December 30, 2015; Accepted: January 5, 2016; Publication Date (Web): January 5, 2016. The authors acknowledge the financial support of the Bill and Melinda Gates Foundation (BMGF OPP1111246). The authors declare no competing financial interest.
Funders:
Funding AgencyGrant Number
Bill and Melinda Gates FoundationOPP1111246
Issue or Number:2
Record Number:CaltechAUTHORS:20160111-103924491
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160111-103924491
Official Citation:Sunlight-Activated Propidium Monoazide Pretreatment for Differentiation of Viable and Dead Bacteria by Quantitative Real-Time Polymerase Chain Reaction Xing Xie, Siwen Wang, Sunny C. Jiang, Janina Bahnemann, and Michael R. Hoffmann Environmental Science & Technology Letters 2016 3 (2), 57-61 DOI: 10.1021/acs.estlett.5b00348
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63542
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:13 Jan 2016 00:15
Last Modified:03 Oct 2019 09:29

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