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A Critical Period for Conversion of Ectodermal Cells to a Neural Crest Fate

Ruffins, Seth and Bronner-Fraser, Marianne (2000) A Critical Period for Conversion of Ectodermal Cells to a Neural Crest Fate. Developmental Biology, 218 (1). pp. 13-20. ISSN 0012-1606. http://resolver.caltech.edu/CaltechAUTHORS:20160112-081714331

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Abstract

Previously, we found that interactions between neural and nonneural ectoderm can generate neural crest cells, with both the ectodermal and the neuroepithelial cells contributing to induced population (M. A. J. Selleck and M. Bronner-Fraser, 1995, Development 121, 525–538). To further characterize the ability of ectodermal cells to form neural crest, we have challenged their normal fate by transplanting them into the neural tube. To ensure that the ectoderm was from nonneural regions, we utilized extraembryonic ectoderm (the proamnion) and transplanted it into the presumptive midbrain of 1.5-day-old chick embryos. We observed that the grafted ectoderm has the capacity to adopt a neural crest fate, responding within a few hours of surgery by turning on neural crest markers HNK-1 and Slug. However, the competence of the ectoderm to respond to neural crest-inducing signals is time limited, declining rapidly in donors older than the 10-somite stage. Similarly, the inductive capacity of the host midbrain declines in a time-dependent fashion. Our results show that extraembryonic ectoderm has the capacity to form neural crest cells given proper inducing signals, expressing both morphological and molecular markers characteristic of neural crest cells.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1006/dbio.1999.9555DOIArticle
http://www.sciencedirect.com/science/article/pii/S0012160699995556PublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 2000 Academic Press. Received for publication September 17, 1999; Revised October 28, 1999; Accepted October 28, 1999. This work was supported by NIH Grants USPHS NS34671 and NS36585 to M.B.F. and NRSA NS11008-1 to S.R.
Funders:
Funding AgencyGrant Number
NIHNS34671
NIHNS36585
National Research Service Award (NRSA)NS11008-1
Subject Keywords:neural crest cells; notochord; Slug; neural plate
Record Number:CaltechAUTHORS:20160112-081714331
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160112-081714331
Official Citation:Seth Ruffins, Marianne Bronner-Fraser, A Critical Period for Conversion of Ectodermal Cells to a Neural Crest Fate, Developmental Biology, Volume 218, Issue 1, 1 February 2000, Pages 13-20, ISSN 0012-1606, http://dx.doi.org/10.1006/dbio.1999.9555. (http://www.sciencedirect.com/science/article/pii/S0012160699995556)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63565
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:12 Jan 2016 22:21
Last Modified:12 Jan 2016 22:21

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