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Role of nerve growth factor in the development of rat sympathetic neurons in vitro. I. Survival, growth, and differentiation of catecholamine production

Chun, Linda L. Y. and Patterson, Paul H. (1977) Role of nerve growth factor in the development of rat sympathetic neurons in vitro. I. Survival, growth, and differentiation of catecholamine production. Journal of Cell Biology, 75 (3). pp. 694-704. ISSN 0021-9525. PMCID PMC2111592. http://resolver.caltech.edu/CaltechAUTHORS:20160113-111831870

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Abstract

The effect of nerve growth factor (NGF) on the development of cholinergic sympathetic neurons was studied in cultures grown either on monolayers of dissociated rat heart cells or in medium conditioned by them. In the presence of rat heart cells the absolute requirement of neurons for exogenous NGF was partially spared. The ability of heart cells to support neuronal survival was due at least in part to production of a diffusable NGF-like substance into the medium. Although some neurons survived on the heart cell monolayer without added NGF, increased levels of exogenous NGF increased neuronal survival until saturation was achieved at 0.5 microgram/ml 7S NGF. The ability of neurons to produce acetylcholine (ACh) from choline was also dependent on the level of exogenous NGF. In mixed neuron-heart cell cultures, NGF increased both ACh and catecholamine (CA) production per neuron to the same extent; saturation occurred at 1 microgram/ml 7S NGF. As cholinergic neurons developed in culture, they became less dependent on NGF for survival and ACh production, but even in older cultures approximately 40% of the neurons died when NGF was withdrawn. Thus, NGF is as necessary for survival, growth, and differentiation of sympathetic neurons when the neurons express cholinergic functions as when the neurons express adrenergic functions.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1083/jcb.75.3.712DOIArticle
http://jcb.rupress.org/content/75/3/712.abstractPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2111592/PubMed CentralArticle
Additional Information:© 1977 Rockefeller University Press. After the Initial Publication Period, RUP will grant to the public the non- exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. Received for publication 9 May 1977. We wish to thank Doreen McDowell and Karen Fischer for help with the culturing, Eleanor Livingston, Shirley Wilson, and Joe Gagliardi for help with the manuscript, and Ed Kravitz for the use of his equipment. This research was supported by a grant-in-aid (93-877) from the American and Massachusetts Heart Associations and a U. S. Public Health Service (PHS) grant (1 RO1 NS 11027) from the National Institute of Neurological and Communicable Diseases and Stroke (NINCDS). L. L. Y. Chun is a PHS trainee (TOl NS 05731), and P. H. Patterson is a research career development awardee of the NINCDS (1 K04 NS 70806).
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)93-877
U.S. Public Health Service (USPHS)1 RO1 NS 11027
U.S. Public Health Service (USPHS)TOl NS 05731
National Institute of Neurological and Communicable Diseases and Stroke (NINCDS)1 K04 NS 70806
American Heart AssociationUNSPECIFIED
Massachusetts Heart AssociationUNSPECIFIED
Subject Keywords:nerve growth factor, sympathetic neurons, cell culture, catecholamine production
PubMed Central ID:PMC2111592
Record Number:CaltechAUTHORS:20160113-111831870
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160113-111831870
Official Citation:Role of nerve growth factor in the development of rat sympathetic neurons in vitro. I. Survival, growth, and differentiation of catecholamine production. L L Chun and P H Patterson 75:694-704. doi:10.1083/jcb.75.3.694
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63633
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:13 Jan 2016 19:54
Last Modified:13 Jan 2016 19:54

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