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Long-term culture of dissociated sympathetic neurons

Hawrot, Edward and Patterson, Paul H. (1979) Long-term culture of dissociated sympathetic neurons. In: Cell Culture. Methods in Enzymology. No.58. New York Academy Press , New York, NY, pp. 574-584. ISBN 978-0-12-181958-3 . http://resolver.caltech.edu/CaltechAUTHORS:20160113-120114770

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Abstract

This chapter discusses long-term primary culture of dissociated neurons, a technique that proved to be a valuable tool in studies of neuronal development and function. Such cultures offer the potential for systematic manipulation of the fluid medium surrounding the cells. These neuron-alone cultures are particularly well suited to biochemical analysis as the properties of any function under study are directly attributable to the neurons being cultured, without complicating contamination from other cell types. In dissociated cell culture under appropriate conditions, sympathetic neurons exhibit a normal developmental differentiation and maturation into adrenergic neurons. Medium conditioned by incubation on cultures of appropriate non-neuronal cells, when added to neuron-alone cultures produces an increase in neuronal cholinergic characteristics with a concomitant decrease in adrenergic characteristics. The extracellular environment is critically important in determining the differentiated fate of sympathetically derived neurons.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0076-6879(79)58174-9DOIArticle
http://www.sciencedirect.com/science/article/pii/S0076687979581749PublisherArticle
Additional Information:© 1979 by Academic Press, Inc. Part of the work described here was supported by the American and Massachusetts Heart Associations, the Helen Hay Whitney Foundation, and the National Institute of Neurological and Communicable Diseases and Stroke.
Funders:
Funding AgencyGrant Number
American Heart AssociationUNSPECIFIED
Helen Hay Whitney FoundationUNSPECIFIED
National Institute of Neurological and Communicable Diseases and Stroke (NINCDS)UNSPECIFIED
Massachusetts Heart AssociationUNSPECIFIED
Record Number:CaltechAUTHORS:20160113-120114770
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160113-120114770
Official Citation:Edward Hawrot, Paul H. Patterson, [53] Long-term culture of dissociated sympathetic neurons, Methods in Enzymology, Academic Press, 1979, Volume 58, Pages 574-584, ISSN 0076-6879, ISBN 9780121819583, http://dx.doi.org/10.1016/S0076-6879(79)58174-9. (http://www.sciencedirect.com/science/article/pii/S0076687979581749)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63636
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:13 Jan 2016 21:17
Last Modified:13 Jan 2016 21:17

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