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The C_2H_2 zinc finger genes of Strongylocentrotus purpuratus and their expression in embryonic development

Materna, Stefan C. and Howard-Ashby, Meredith and Gray, Rachel F. and Davidson, Eric H. (2006) The C_2H_2 zinc finger genes of Strongylocentrotus purpuratus and their expression in embryonic development. Developmental Biology, 300 (1). pp. 108-120. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20160114-071949379

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Abstract

The C_2H_2 zinc finger is one of the most abundant protein domains and is thought to have been extensively replicated in diverse animal clades. Some well-studied proteins that contain this domain are transcriptional regulators. As part of an attempt to delineate all transcription factors encoded in the Strongylocentrotus purpuratus genome, we identified the C_2H_2 zinc finger genes indicated in the sequence, and examined their involvement in embryonic development. We found 377 zinc finger genes in the sea urchin genome, about half the number found in mice or humans. Their expression was measured by quantitative PCR. Up to the end of gastrulation less than a third of these genes is expressed, and about 75% of the expressed genes are maternal; both parameters distinguish these from all other classes of regulatory genes as measured in other studies. Spatial expression pattern was determined by whole mount in situ hybridization for 43 genes transcribed at a sufficient level, and localized expression was observed in diverse embryonic tissues. These genes may execute important regulatory functions in development. However, the functional meaning of the majority of this large gene family remains undefined.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.ydbio.2006.08.032DOIArticle
http://www.sciencedirect.com/science/article/pii/S001216060601102XPublisherArticle
Additional Information:© 2006 Elsevier Inc. Received for publication 25 May 2006; revised 4 August 2006; accepted 16 August 2006. Available online 22 August 2006. The authors would like to thank C.T. Brown for help with computation, T. Minokawa for initial help with WMISH, and J. Nam for critical support of phylogenetic analysis. We are grateful for helpful comments and suggestions on the manuscript by V. Hinman and J. Nam. This research was supported by NIH grant HD37105 and by the Office of Science (BER), US Department of Energy, grant DE-FG02-03ER63584.
Funders:
Funding AgencyGrant Number
NIHHD37105
Department of Energy (DOE)DE-FG02-03ER63584
Subject Keywords:Zinc finger; Zf-c2h2; Transcription factor; Genome; Sea urchin; Development
Issue or Number:1
Record Number:CaltechAUTHORS:20160114-071949379
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160114-071949379
Official Citation:Stefan C. Materna, Meredith Howard-Ashby, Rachel F. Gray, Eric H. Davidson, The C2H2 zinc finger genes of Strongylocentrotus purpuratus and their expression in embryonic development, Developmental Biology, Volume 300, Issue 1, 1 December 2006, Pages 108-120, ISSN 0012-1606, http://dx.doi.org/10.1016/j.ydbio.2006.08.032. (http://www.sciencedirect.com/science/article/pii/S001216060601102X)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63664
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:14 Jan 2016 19:38
Last Modified:03 Oct 2019 09:30

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