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Both neural crest and placode contribute to the ciliary ganglion and oculomotor nerve

Lee, Vivian M. and Sechrist, John W. and Luetolf, Simone and Bronner-Fraser, Marianne (2003) Both neural crest and placode contribute to the ciliary ganglion and oculomotor nerve. Developmental Biology, 263 (2). pp. 176-190. ISSN 0012-1606. doi:10.1016/j.ydbio.2003.07.004.

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The chick ciliary ganglion is a neural crest-derived parasympathetic ganglion that innervates the eye. Here, we examine its axial level of origin and developmental relationship to other ganglia and nerves of the head. Using small, focal injections of DiI, we show that neural crest cells arising from both the caudal half of the midbrain and the rostral hindbrain contribute to the ciliary as well as the trigeminal ganglion. Precursors to both ganglia have overlapping migration patterns, moving first ventrolaterally and then rostrally toward the optic vesicle. At the level of the midbrain/forebrain junction, precursors to the ciliary ganglion separate from the main migratory stream, turn ventromedially, and condense in the vicinity of the rostral aorta and Rathke's pouch. Ciliary neuroblasts first exit the cell cycle at early E2, prior to and during ganglionic condensation, and neurogenesis continues through E5.5. By E3, markers of neuronal differentiation begin to appear in this population. By labeling the ectoderm with DiI, we discovered a new placode, caudal to the eye and possibly contiguous to the trigeminal placode, that contributes a few early differentiating neurons to the ciliary ganglion, oculomotor nerve, and connecting branches to the ophthalmic nerve. These results suggest for the first time a dual neural crest and placodal contribution to the ciliary ganglion and associated nerves.

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Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 2003 Elsevier Inc. Received for publication 11 March 2003, revised 28 July 2003, accepted 28 July 2003. We thank Dr. Louis Reichardt and Dr. Virginia Lee for their generous gifts of antibodies. We thank Dr. Meyer Barenbaum for sharing his unpublished results. This work is supported by USPHS Grant NS41070 (to M.B.F.) and an American Heart Association postdoctoral fellowship (to V.M.L.).
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American Heart AssociationUNSPECIFIED
Subject Keywords:Chick; DiI; Migration; Neuronal differentiation; Neurogenesis; Ophthalmic nerve; Trigeminal ganglion
Issue or Number:2
Record Number:CaltechAUTHORS:20160114-071950620
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Official Citation:Vivian M Lee, John W Sechrist, Simone Luetolf, Marianne Bronner-Fraser, Both neural crest and placode contribute to the ciliary ganglion and oculomotor nerve, Developmental Biology, Volume 263, Issue 2, 15 November 2003, Pages 176-190, ISSN 0012-1606, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63667
Deposited By: Ruth Sustaita
Deposited On:14 Jan 2016 19:48
Last Modified:10 Nov 2021 23:19

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