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Correct Expression of spec2a in the Sea Urchin Embryo Requires Both Otx and Other cis-Regulatory Elements

Yuh, Chiou-Hwa and Li, Xiaotao and Davidson, Eric H. and Klein, William H. (2001) Correct Expression of spec2a in the Sea Urchin Embryo Requires Both Otx and Other cis-Regulatory Elements. Developmental Biology, 232 (2). pp. 424-438. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20160114-082654848

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Abstract

Strongylocentrotus purpuratus Otx (SpOtx) is required simultaneously in sea urchin development for the activation of endo16 in the vegetal plate and for the activation of spec2a in the aboral ectoderm. Because Otx binding sites alone do not appear to be responsible for the spatially restricted expression of spec2a, additional DNA elements were sought. We show here that consensus Otx binding sites fused to basal promoters are sufficient to activate CAT reporter gene expression in all cell types, although expression in endomesoderm progenitors is enhanced. On the other hand, three non-Otx elements derived from the spec2a enhancer are needed together with Otx sites for specifically aboral ectoderm expression. A DNA element termed Y/CBF, lying just downstream from an Otx site within the spec2a enhancer, mediates general activation in the ectoderm. A second element lying between the Otx and Y/CBF sites, called OER, functions to prevent expression in the oral ectoderm. A third site, called ENR, overlapping another Otx site, is required to repress endoderm expression. Three distinct DNA binding proteins interact sequence specifically at the Y/CBF, OER, and ENR elements. The spec2a enhancer thus consists of closely linked activator and repressor elements that function collectively to cause expression of the spec2a gene in the aboral ectoderm.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1006/dbio.2001.0207DOIArticle
http://www.sciencedirect.com/science/article/pii/S0012160601902076PublisherArticle
Additional Information:© 2001 Academic Press. Received for publication December 1, 2000. Accepted January 19, 2001. Published online March 16, 2001. This work was supported by the Stowers Institute for Medical Research, by an NIH grant to E.H.D. (HD37105) and an NIH grant to W.H.K. (HD22619), by the Welch Foundation (W.H.K.), and by M. D. Anderson Cancer Center NCI Core Grant CA16672.
Funders:
Funding AgencyGrant Number
Stowers Institute for Medical ResearchUNSPECIFIED
NIHHD37105
NIHHD22619
Welch FoundationUNSPECIFIED
National Cancer InstituteCA16672
Subject Keywords:Strongylocentrotus purpuratus; sea urchin development; Otx proteins; CAAT binding factor; endoderm and ectoderm differentiation
Issue or Number:2
Record Number:CaltechAUTHORS:20160114-082654848
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160114-082654848
Official Citation:Chiou-Hwa Yuh, Xiaotao Li, Eric H Davidson, William H Klein, Correct Expression of spec2a in the Sea Urchin Embryo Requires Both Otx and Other cis-Regulatory Elements, Developmental Biology, Volume 232, Issue 2, 15 April 2001, Pages 424-438, ISSN 0012-1606, http://dx.doi.org/10.1006/dbio.2001.0207. (http://www.sciencedirect.com/science/article/pii/S0012160601902076)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63672
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:14 Jan 2016 19:27
Last Modified:03 Oct 2019 09:30

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