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Relationship between Gene Expression Domains of Xsnail, Xslug, and Xtwist and Cell Movement in the Prospective Neural Crest of Xenopus

Linker, Claudia and Bronner-Fraser, Marianne and Mayor, Roberto (2000) Relationship between Gene Expression Domains of Xsnail, Xslug, and Xtwist and Cell Movement in the Prospective Neural Crest of Xenopus. Developmental Biology, 224 (2). pp. 215-225. ISSN 0012-1606. http://resolver.caltech.edu/CaltechAUTHORS:20160114-082655382

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Abstract

The markers Xslug, Xsnail, and Xtwist all are expressed in the presumptive neural folds and are thought to delineate the presumptive neural crest. However, their interrelationship and relative spatiotemporal distributions are not well understood. Here, we present a detailed in situ hybridization analysis of the relative patterns of expression of these transcription factors from gastrulation through neurulation and post-neural crest migration. The three genes mark the prospective neural crest and roof plate, coming on sequentially, with Xsnail preceding Xslug preceding Xtwist. By combining gene expression analysis with a fate map of the same region using DiI labeling, we determined the correspondence between early and late domains of gene expression. At the beginning of gastrulation, Xsnail is present in a unique domain of expression in a lateral region of the embryo in both superficial and deep layers of the ectoderm, as are Xslug and Xtwist. During gastrulation and neurulation, the superficial layer moves faster toward the dorsal midline than the deep layer, producing a relative shift in these cell populations. By early neurula stage, the Xsnail domain is split into a medial domain in the superficial ectoderm (fated to become the roof plate) and a lateral domain in the deep layer of the ectoderm (fated to become neural crest). Xsnail is down-regulated in the most anterior neural plate and up-regulated in the posterior neural plate. Our results show that changes in the expression of Xsnail, Xslug, and Xtwist are a consequence of active cell movement in some regions coupled with dynamic changes in gene expression in other regions.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1006/dbio.2000.9723DOIArticle
http://www.sciencedirect.com/science/article/pii/S0012160600997239PublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 2000 Academic Press. Received 8 December 1999, Revised 28 March 2000, Accepted 28 March 2000, Available online 25 March 2002. We thank Drs. P. Kulesa, G. Belford, A. Collazo, S. Ruffin, and S. Fraser for their kind help with image processing and analysis. We are very grateful to Dr. F. Bru¨ cher for his computational advice. We thank Dr. S. He-Kee for her extensive help with videomicroscopy. We thank Dr. N. Papalopulu for her helpful recommendations for the in situ hybridization in sections, Dr. J. Gurdon for providing us with Xtwist probe, and Dr. K. Dale for her comments on the manuscript. Special thanks to the members of the Bronner-Fraser laboratory for their kindness and in particular to Ella Moreno for embryological expertise. This investigation was supported by grants from Fondecyt 1990570, by the Universidad de Chile, by NSF Grant INT-9722460, and by USPHS NS36585; C.L. was funded by Fundacion Andes.
Funders:
Funding AgencyGrant Number
Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT)1990570
NSFINT-9722460
NIHNS36585
Universidad de ChileUNSPECIFIED
Fundacion AndesUNSPECIFIED
Subject Keywords:gastrulation; neurulation; DiI; neural crest
Record Number:CaltechAUTHORS:20160114-082655382
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160114-082655382
Official Citation:Claudia Linker, Marianne Bronner-Fraser, Roberto Mayor, Relationship between Gene Expression Domains of Xsnail, Xslug, and Xtwist and Cell Movement in the Prospective Neural Crest of Xenopus, Developmental Biology, Volume 224, Issue 2, 15 August 2000, Pages 215-225, ISSN 0012-1606, http://dx.doi.org/10.1006/dbio.2000.9723. (http://www.sciencedirect.com/science/article/pii/S0012160600997239)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63674
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:14 Jan 2016 19:31
Last Modified:14 Jan 2016 19:31

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