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Evidence for a Mesodermal Embryonic Regulator of the Sea Urchin CyIIa Gene

Martin, E. L. and Consales, C. and Davidson, E. H. and Arnone, M. I. (2001) Evidence for a Mesodermal Embryonic Regulator of the Sea Urchin CyIIa Gene. Developmental Biology, 236 (1). pp. 46-63. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20160114-101925387

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Abstract

The CyIIa gene of the sea urchin embryo is a model for study of cis-regulation downstream of cell-type specification, as CyIIa transcription follows the specification and initial differentiation of the embryonic domains in which it is expressed. These are the skeletogenic and secondary mesenchyme and gut. We carried out a detailed structural and functional analysis of a cis-regulatory region of this gene, extending 780 bp upstream and 125 bp downstream of the transcription start site, that had been shown earlier to reproduce faithfully the complex and dynamic CyIIa pattern of expression. This analysis revealed that the overall pattern of expression of the CyIIa gene appears to be governed mainly by two independent sets of DNA elements, which are target sites for specific proteins present in blastula-stage nuclear extract. One type of element, which controls a dynamic program of expression in both skeletogenic and secondary mesenchyme cells, contains the consensus-binding site for a member of the ets transcription factor family. The other, which is responsible for the terminal or permanent phase of CyIIa expression in the gut, shares homologies with the late module of the endoderm-specific Endo16 gene (endo16 Module B). Oligonucleotides containing replicas of these two target sites fused upstream of a sea urchin basal promoter are sufficient to confer accurate mesenchyme and late gut expression of an injected GFP construct. The finding of a single protein target site that recapitulates CyIIa expression in both primary and secondary mesenchyme cells suggests the existence of a pan-mesodermal gene expression program in the sea urchin embryo.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1006/dbio.2001.0285DOIArticle
http://www.sciencedirect.com/science/article/pii/S0012160601902854PublisherArticle
Additional Information:© 2001 Academic Press. Received for publication February 15, 2001. Revised April 3, 2001. Accepted April 3, 2001. Published online June 22, 2001. We thank Jong Tai Chun and Caterina Missero at Stazione Zoologica, and Chiou-Hwa Yuh and Paola Oliveri at California Institute of Technology for critical review of the manuscript. This work was partially supported by the Stowers Institute for Medical Research. E.L.M. was supported by the National Institute of Child Health and Human Development, Grant HD37105 (to E.H.D.).
Funders:
Funding AgencyGrant Number
Stowers Institute for Medical ResearchUNSPECIFIED
National Institute of Child Health and Human Development (NICHD)HD37105
Subject Keywords:gene regulation; sea urchin embryo; CyIIa; mesenchyme cells regulator; ets.
Issue or Number:1
Record Number:CaltechAUTHORS:20160114-101925387
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160114-101925387
Official Citation:E.L Martin, C Consales, E.H Davidson, M.I Arnone, Evidence for a Mesodermal Embryonic Regulator of the Sea Urchin CyIIa Gene, Developmental Biology, Volume 236, Issue 1, 1 August 2001, Pages 46-63, ISSN 0012-1606, http://dx.doi.org/10.1006/dbio.2001.0285. (http://www.sciencedirect.com/science/article/pii/S0012160601902854)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63681
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:14 Jan 2016 19:22
Last Modified:03 Oct 2019 09:30

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