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Identification and Characterization of a Calcium Channel γ Subunit Expressed in Differentiating Neurons and Myoblasts

Kious, Brent M. and Baker, Clare V. H. and Bronner-Fraser, Marianne and Knecht, Anne K. (2002) Identification and Characterization of a Calcium Channel γ Subunit Expressed in Differentiating Neurons and Myoblasts. Developmental Biology, 243 (2). pp. 249-259. ISSN 0012-1606. doi:10.1006/dbio.2001.0570. https://resolver.caltech.edu/CaltechAUTHORS:20160114-101925932

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Abstract

Transient elevations of intracellular calcium (calcium transients) play critical roles in many developmental processes, including differentiation. Although the factors that regulate calcium transients are not clearly defined, calcium influx may be controlled by molecules interacting with calcium channels, including channel regulatory subunits. Here, we describe the chick γ4 regulatory subunit (CACNG4), the first such subunit to be characterized in early development. CACNG4 is expressed early in the cranial neural plate, and later in the cranial and dorsal root ganglia; importantly, the timing of this later expression correlates precisely with the onset of neuronal differentiation. CACNG4 expression is also observed in nonneuronal tissues undergoing differentiation, specifically the myotome and a subpopulation of differentiating myoblasts in the limb bud. Finally, within the distal cranial ganglia, we show that CACNG4 is expressed in placode-derived cells (prospective neurons), but also, surprisingly, in neural crest-derived cells, previously shown to form only glia in this location; contrary to these previous results, we find that neural crest cells can form neurons in the distal ganglia. Given the proposed role of CACNG4 in modulating calcium channels and its expression in differentiating cells, we suggest that CACNG4 may promote differentiation via regulation of intracellular calcium levels.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1006/dbio.2001.0570DOIArticle
http://www.sciencedirect.com/science/article/pii/S0012160601905706PublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 2002 Elsevier Science (USA). Received for publication September 14, 2001. Revised December 4, 2001. Accepted December 4, 2001. Published online February 11, 2002. We thank Yun Kee for the chick embryo library, Jack Sechrist for assistance with anatomical identification, and Helen McBride and Tanya Moreno for critical reading of the manuscript. This work was supported by grants from the NIH (DE13223 and NS41070) to M.B.F.; B.M.K. was supported by an undergraduate research fellowship from the Arnold and Mabel Beckman Foundation; C.V.H.B. was supported by American Heart Association Fellowships 1171-F11 and 0020097Y; and A.K.K. was supported by postdoctoral fellowships from the Cancer Research Fund of the Damon Runyon-Walter Winchell Foundation, and from the NIH (DE14134).
Funders:
Funding AgencyGrant Number
NIHDE13223
NIHNS41070
Arnold and Mabel Beckman FoundationUNSPECIFIED
American Heart Association1171-F11
American Heart Association0020097Y
Damon Runyon-Walter Winchell Foundation Cancer Research FundUNSPECIFIED
NIHDE14134
Subject Keywords:CACNG4 (γ4); calcium channel; neuronal differentiation; myoblasts; cranial ganglia; placodes
Issue or Number:2
DOI:10.1006/dbio.2001.0570
Record Number:CaltechAUTHORS:20160114-101925932
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160114-101925932
Official Citation:Brent M. Kious, Clare V.H. Baker, Marianne Bronner-Fraser, Anne K. Knecht, Identification and Characterization of a Calcium Channel γ Subunit Expressed in Differentiating Neurons and Myoblasts, Developmental Biology, Volume 243, Issue 2, 15 March 2002, Pages 249-259, ISSN 0012-1606, http://dx.doi.org/10.1006/dbio.2001.0570. (http://www.sciencedirect.com/science/article/pii/S0012160601905706)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63683
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:14 Jan 2016 19:26
Last Modified:10 Nov 2021 23:20

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