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Pax3-Expressing Trigeminal Placode Cells Can Localize to Trunk Neural Crest Sites but Are Committed to a Cutaneous Sensory Neuron Fate

Baker, Clare V. H. and Stark, Michael R. and Bronner-Fraser, Marianne (2002) Pax3-Expressing Trigeminal Placode Cells Can Localize to Trunk Neural Crest Sites but Are Committed to a Cutaneous Sensory Neuron Fate. Developmental Biology, 249 (2). pp. 219-236. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20160121-075839528

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Abstract

The cutaneous sensory neurons of the ophthalmic lobe of the trigeminal ganglion are derived from two embryonic cell populations, the neural crest and the paired ophthalmic trigeminal (opV) placodes. Pax3 is the earliest known marker of opV placode ectoderm in the chick. Pax3 is also expressed transiently by neural crest cells as they emigrate from the neural tube, and it is reexpressed in neural crest cells as they condense to form dorsal root ganglia and certain cranial ganglia, including the trigeminal ganglion. Here, we examined whether Pax3+ opV placode-derived cells behave like Pax3+ neural crest cells when they are grafted into the trunk. Pax3+ quail opV ectoderm cells associate with host neural crest migratory streams and form Pax3+ neurons that populate the dorsal root and sympathetic ganglia and several ectopic sites, including the ventral root. Pax3 expression is subsequently downregulated, and at E8, all opV ectoderm-derived neurons in all locations are large in diameter, and virtually all express TrkB. At least some of these neurons project to the lateral region of the dorsal horn, and peripheral quail neurites are seen in the dermis, suggesting that they are cutaneous sensory neurons. Hence, although they are able to incorporate into neural crest-derived ganglia in the trunk, Pax3+ opV ectoderm cells are committed to forming cutaneous sensory neurons, their normal fate in the trigeminal ganglion. In contrast, Pax3 is not expressed in neural crest-derived neurons in the dorsal root and trigeminal ganglia at any stage, suggesting either that Pax3 is expressed in glial cells or that it is completely downregulated before neuronal differentiation. Since Pax3 is maintained in opV placode-derived neurons for some considerable time after neuronal differentiation, these data suggest that Pax3 may play different roles in opV placode cells and neural crest cells.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1006/dbio.2002.0767DOIArticle
http://www.sciencedirect.com/science/article/pii/S0012160602907670PublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 2002 Elsevier Science. Received for publication May 6, 2002. Revised June 24, 2002. Published online August 13, 2002. We thank Dr. Louis Reichardt for the anti-TrkB antibody and Dr. Hideaki Tanaka for the QN antibody. Thanks to Drs. Andy Groves and Roger Keynes for valuable comments on the manuscript. This work was supported by NS-041070–02 from the NIH (to M.B.-F.) and by Human Frontier Science Program Fellowship LT-63/96 and American Heart Association Fellowships 1171-FI1 and 0020097Y (to C.V.H.B.).
Funders:
Funding AgencyGrant Number
NIHNS-041070–02
Human Frontier Science ProgramLT-63/96
American Heart Association1171-FI1
American Heart Association0020097Y
Issue or Number:2
Record Number:CaltechAUTHORS:20160121-075839528
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160121-075839528
Official Citation:Clare V.H Baker, Michael R Stark, Marianne Bronner-Fraser, Pax3-Expressing Trigeminal Placode Cells Can Localize to Trunk Neural Crest Sites but Are Committed to a Cutaneous Sensory Neuron Fate, Developmental Biology, Volume 249, Issue 2, 15 September 2002, Pages 219-236, ISSN 0012-1606, http://dx.doi.org/10.1006/dbio.2002.0767. (http://www.sciencedirect.com/science/article/pii/S0012160602907670)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63823
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:21 Jan 2016 18:54
Last Modified:03 Oct 2019 09:32

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