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Neuronal Differentiation from Postmitotic Precursors in the Ciliary Ganglion

Lee, Vivian M. and Sechrist, John W. and Bronner-Fraser, Marianne and Nishi, Rae (2002) Neuronal Differentiation from Postmitotic Precursors in the Ciliary Ganglion. Developmental Biology, 252 (2). pp. 312-323. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20160121-075839812

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Abstract

In the chick ciliary ganglion, neuronal number is kept constant between St. 29 and St. 34 (E6–E8) despite a large amount of cell death. Here, we characterize the source of neurogenic cells in the ganglion as undifferentiated neural crest-derived cells. At St. 29, neurons and nonneuronal cells in the ciliary ganglion expressed the neural crest markers HNK-1 and p75^(NTR). Over 50% of the cells were neurons at St. 29; of the nonneuronal cells, a small population expressed glial markers, whereas the majority was undifferentiated. When placed in culture, nonneuronal cells acquired immunoreactivity for HuD, suggesting that they had commenced neuronal differentiation. The newly differentiated neurons arose from precursors that did not incorporate bromodeoxyuridine. To test whether these precursors could undergo neural differentiation in vivo, purified nonneuronal cells from St. 29 quail ganglia were transplanted into chick embryos at St. 9–14. Subsequently, quail cells expressing neuronal markers were found in the chick ciliary ganglion. The existence of this precursor pool was transient because nonneuronal cells isolated from St. 38 ganglia failed to form neurons. Since all ciliary ganglion neurons are born prior to St. 29, these results demonstrate that there are postmitotic neural crest-derived precursors in the developing ciliary ganglion that can differentiate into neurons in the appropriate environment.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1006/dbio.2002.0882DOIArticle
http://www.sciencedirect.com/science/article/pii/S0012160602908821PublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 2002 Elsevier Science. Received for publication December 28, 2001. Revised October 11, 2002. Accepted October 14, 2002. Published online November 19, 2002. We thank Greg Smiley and Fanny Vang for technical assistance. We are also grateful to Dr. Jim Weston and Dr. Mike Marusich for their generous gifts of antibodies. We thank Dr. Lou Reichardt for supplying us with the anti-p75 neurotrophin receptor. We are also indebted to Dr. Gary Banker and his laboratory for the use of their imaging setup. We also thank Dan Darcy at the Caltech BioImaging Center for assistance and training of confocal microscopy. This study was funded by NS25767 (to R.N.), NS41070 (to J.W.S. and M.B.F.), and N. L. Tartar fellowship (to V.M.L.).
Funders:
Funding AgencyGrant Number
NIHNS25767
NIHNS41070
N. L. Tartar FellowshipUNSPECIFIED
Subject Keywords:ciliary ganglion; parasympathetic; neuronal differentiation; quail; Islet-1; HuD; transplantation; neurogenesis; neural crest
Issue or Number:2
Record Number:CaltechAUTHORS:20160121-075839812
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160121-075839812
Official Citation:Vivian M. Lee, John W. Sechrist, Marianne Bronner-Fraser, Rae Nishi, Neuronal Differentiation from Postmitotic Precursors in the Ciliary Ganglion, Developmental Biology, Volume 252, Issue 2, 15 December 2002, Pages 312-323, ISSN 0012-1606, http://dx.doi.org/10.1006/dbio.2002.0882. (http://www.sciencedirect.com/science/article/pii/S0012160602908821)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63824
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:21 Jan 2016 18:56
Last Modified:03 Oct 2019 09:32

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