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Combined intrinsic and extrinsic influences pattern cranial neural crest migration and pharyngeal arch morphogenesis in axolotl

Cerny, Robert and Meulemans, Daniel and Berger, Jürgen and Wilsch-Bräuninger, Michaela and Kurth, Thomas and Bronner-Fraser, Marianne and Epperlein, Hans-Henning (2004) Combined intrinsic and extrinsic influences pattern cranial neural crest migration and pharyngeal arch morphogenesis in axolotl. Developmental Biology, 266 (2). pp. 252-269. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20160121-103518548

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Abstract

Cranial neural crest cells migrate in a precisely segmented manner to form cranial ganglia, facial skeleton and other derivatives. Here, we investigate the mechanisms underlying this patterning in the axolotl embryo using a combination of tissue culture, molecular markers, scanning electron microscopy and vital dye analysis. In vitro experiments reveal an intrinsic component to segmental migration; neural crest cells from the hindbrain segregate into distinct streams even in the absence of neighboring tissue. In vivo, separation between neural crest streams is further reinforced by tight juxtapositions that arise during early migration between epidermis and neural tube, mesoderm and endoderm. The neural crest streams are dense and compact, with the cells migrating under the epidermis and outside the paraxial and branchial arch mesoderm with which they do not mix. After entering the branchial arches, neural crest cells conduct an “outside-in” movement, which subsequently brings them medially around the arch core such that they gradually ensheath the arch mesoderm in a manner that has been hypothesized but not proven in zebrafish. This study, which represents the most comprehensive analysis of cranial neural crest migratory pathways in any vertebrate, suggests a dual process for patterning the cranial neural crest. Together with an intrinsic tendency to form separate streams, neural crest cells are further constrained into channels by close tissue apposition and sorting out from neighboring tissues.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.ydbio.2003.09.039DOIArticle
http://www.sciencedirect.com/science/article/pii/S0012160603006067PublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 2003 Elsevier Inc. Received for publication 10 May 2003, revised 15 August 2003, accepted 9 September 2003. Available online 4 December 2003. We thank Tanya Moreno for the GFP mRNA, Irmin Beck for help in artwork, and Torsten Schwalm and Lewan Mtschedlischwili for technical advice. Support from NIH grant HD15527 and NASA grant NAG 2-1585 to M.B.F. and Herbert-Quandt Stiftung, SMWK, COST B-23, MSMT grant 1311004 and a NATO Science Fellowships Programme to R.C.
Funders:
Funding AgencyGrant Number
NIHHD15527
NASANAG 2-1585
Herbert-Quandt StiftungSMWK, COST B-23
Ministry of Education, Youth and Sports of the Czech Republic (MŠMT)1311004
North Atlantic Treaty Organization (NATO)UNSPECIFIED
Subject Keywords:Axolotl; Head; Cranial neural crest; Migration; Branchial arches; AP-2; Snail
Issue or Number:2
Record Number:CaltechAUTHORS:20160121-103518548
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160121-103518548
Official Citation:Robert Cerny, Daniel Meulemans, Jürgen Berger, Michaela Wilsch-Bräuninger, Thomas Kurth, Marianne Bronner-Fraser, Hans-Henning Epperlein, Combined intrinsic and extrinsic influences pattern cranial neural crest migration and pharyngeal arch morphogenesis in axolotl, Developmental Biology, Volume 266, Issue 2, 15 February 2004, Pages 252-269, ISSN 0012-1606, http://dx.doi.org/10.1016/j.ydbio.2003.09.039. (http://www.sciencedirect.com/science/article/pii/S0012160603006067)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63832
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:21 Jan 2016 20:22
Last Modified:03 Oct 2019 09:32

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