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SpHnf6, a transcription factor that executes multiple functions in sea urchin embryogenesis

Otim, Ochan and Amore, Gabriele and Minokawa, Takuya and McClay, David R. and Davidson, Eric H. (2004) SpHnf6, a transcription factor that executes multiple functions in sea urchin embryogenesis. Developmental Biology, 273 (2). pp. 226-243. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20160121-103519400

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Abstract

The Strongylocentrotus purpuratus hnf6 (Sphnf6) gene encodes a new member of the ONECUT family of transcription factors. The expression of hnf6 in the developing embryo is triphasic, and loss-of-function analysis shows that the Hnf6 protein is a transcription factor that has multiple distinct roles in sea urchin development. hnf6 is expressed maternally, and before gastrulation its transcripts are distributed globally. Early in development, its expression is required for the activation of PMC differentiation genes such as sm50, pm27, and msp130, but not for the activation of any known PMC regulatory genes, for example, alx, ets1, pmar1, or tbrain. Micromere transplantation experiments show that the gene is not involved in early micromere signaling. Early hnf6 expression is also required for expression of the mesodermal regulator gatac. The second known role of hnf6 is its participation after gastrulation in the oral ectoderm gene regulatory network (GRN), in which its expression is essential for the maintenance of the state of oral ectoderm specification. The third role is in the neurogenic ciliated band, which is foreshadowed exactly by a trapezoidal band of hnf6 expression at the border of the oral ectoderm and where it continues to be expressed through the end of embryogenesis. Neither oral ectoderm regulatory functions nor ciliated band formation occur normally in the absence of hnf6 expression.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.ydbio.2004.05.033DOIArticle
http://www.sciencedirect.com/science/article/pii/S0012160604004129PublisherArticle
ORCID:
AuthorORCID
Otim, Ochan0000-0001-7272-4356
Additional Information:© 2004 Elsevier Inc. Received for publication 21 January 2004, revised 21 May 2004, accepted 21 May 2004. Available online 20 July 2004. We would like to acknowledge technical support this work received from LijunWang of Oxford University during her 2002 Summer Scholarship in our lab, Miki Yun, and Sagar Damle of this laboratory. The first author would like in addition to thank Dr. James A. Coffman of Stowers Institute of Medical Research, Kansas City, MO, and Dr. Zeev Pancer of University of Alabama at Birmingham for useful discussions during the early part of this work. This work was supported by NIH Grant HD37105; by the Office of Science (BER), U.S. Department of Energy, Grant No. DEFG02-03ER63584; and by the Caltech Beckman Institute.
Funders:
Funding AgencyGrant Number
NIHHD37105
Department of Energy (DOE)DE-FG02-03ER63584
Caltech Beckman InstituteUNSPECIFIED
Subject Keywords:Morpholino antisense oligonucleotide; Transcription factor; hnf6; ONECUT; Oral ectoderm; PMC; Sea urchin embryo
Issue or Number:2
Record Number:CaltechAUTHORS:20160121-103519400
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160121-103519400
Official Citation:Ochan Otim, Gabriele Amore, Takuya Minokawa, David R. McClay, Eric H. Davidson, SpHnf6, a transcription factor that executes multiple functions in sea urchin embryogenesis, Developmental Biology, Volume 273, Issue 2, 15 September 2004, Pages 226-243, ISSN 0012-1606, http://dx.doi.org/10.1016/j.ydbio.2004.05.033. (http://www.sciencedirect.com/science/article/pii/S0012160604004129)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63835
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:21 Jan 2016 19:48
Last Modified:03 Oct 2019 09:32

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