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Abnormalities in neural crest cell migration in laminin α5 mutant mice

Coles, Edward G. and Gammill, Laura S. and Miner, Jeffrey H. and Bronner-Fraser, Marianne (2006) Abnormalities in neural crest cell migration in laminin α5 mutant mice. Developmental Biology, 289 (1). pp. 218-228. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20160121-135400492

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Abstract

Although numerous in vitro experiments suggest that extracellular matrix molecules like laminin can influence neural crest migration, little is known about their function in the embryo. Here, we show that laminin α5, a gene up-regulated during neural crest induction, is localized in regions of newly formed cranial and trunk neural folds and adjacent neural crest migratory pathways in a manner largely conserved between chick and mouse. In laminin α5 mutant mice, neural crest migratory streams appear expanded in width compared to wild type. Conversely, neural folds exposed to laminin α5 in vitro show a reduction by half in the number of migratory neural crest cells. During gangliogenesis, laminin α5 mutants exhibit defects in condensing cranial sensory and trunk sympathetic ganglia. However, ganglia apparently recover at later stages. These data suggest that the laminin α5 subunit functions as a cue that restricts neural crest cells, focusing their migratory pathways and condensation into ganglia. Thus, it is required for proper migration and timely differentiation of some neural crest populations.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.ydbio.2005.10.031DOIArticle
http://www.sciencedirect.com/science/article/pii/S0012160605007591PublisherArticle
ORCID:
AuthorORCID
Miner, Jeffrey H.0000-0002-1510-8714
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 2005 Elsevier Inc. Received for publication 14 June 2005, revised 11 October 2005, accepted 16 October 2005. Available online 28 November 2005. This work was supported by grants from the American Heart Association (0525037Y to E.G.C.) and NIH (DE15309 to L.S.G. and NS051051 to M.B.F.). We would like to thank David Anderson for the mouse NeuroD probe, Kirsten Kuhlbrodt and Michael Wegner for the mouse Sox10, Lou Reichardt for the anti-p75 antibody, Peter Lwigale for the critical reading of the manuscript and Joaquin Gutierrez for the excellent animal husbandry.
Funders:
Funding AgencyGrant Number
American Heart Association0525037Y
NIHDE15309
NIHNS051051
Subject Keywords:Extracellular matrix; Neural crest; Chick; Mouse; Laminin; Ganglia; Cell migration
Issue or Number:1
Record Number:CaltechAUTHORS:20160121-135400492
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160121-135400492
Official Citation:Edward G. Coles, Laura S. Gammill, Jeffrey H. Miner, Marianne Bronner-Fraser, Abnormalities in neural crest cell migration in laminin α5 mutant mice, Developmental Biology, Volume 289, Issue 1, 1 January 2006, Pages 218-228, ISSN 0012-1606, http://dx.doi.org/10.1016/j.ydbio.2005.10.031. (http://www.sciencedirect.com/science/article/pii/S0012160605007591)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63846
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:22 Jan 2016 22:32
Last Modified:09 Mar 2020 13:19

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