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Formation of the dorsal marginal zone in Xenopus laevis analyzed by time-lapse microscopic magnetic resonance imaging

Papan, Cyrus and Boulat, Benoit and Velan, S. Sendhil and Fraser, Scott E. and Jacobs, Russell E. (2007) Formation of the dorsal marginal zone in Xenopus laevis analyzed by time-lapse microscopic magnetic resonance imaging. Developmental Biology, 305 (1). pp. 161-171. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20160121-135402120

[img] Video (MPEG) (Movie 1. 2D MRI time-lapse sequence showing the morphogenetic tissue movements of the Xenopus embryo from early blastula to about stage 10.5) - Supplemental Material
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[img] Video (MPEG) (Movie 2. 2D MRI time-lapse movie showing the movement of a B1-clone from mid-blastula to about stage 10.5.) - Supplemental Material
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[img] Video (MPEG) (Movie 3. 3D MRI time-lapse movie showing the movement of a C1-clone from mid-blastula to about stage 10.5) - Supplemental Material
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Abstract

The dorsal marginal zone (DMZ) of the amphibian embryo is a key embryonic region involved in body axis organization and neural induction. Using time-lapse microscopic magnetic resonance imaging (MRI), we follow the pregastrula movements that lead to the formation of the DMZ of the stage 10 Xenopus embryo. 2D and 3D MRI time-lapse series reveal that pregastrular movements change the tissue architecture of the DMZ at earlier stages and in a different fashion than previously appreciated. Beginning at stage 9, epiboly of the animal cap moves tissue into the dorsal but not into the ventral marginal zone, resulting in an asymmetry between the dorsal and the ventral sides. Time-lapse imaging of labeled blastomeres shows that the animal cap tissue moves into the superficial DMZ overlying the deeper mesendoderm of the DMZ. The shearing of superficial tissue over the deeper mesendoderm creates the radial/vertical arrangement of ectoderm outside of mesendoderm within the DMZ, which is independent of involution and prior to the formation of the dorsal blastoporal lip. This tilting of the DMZ is distinct from, but occurs synchronously with, the vegetal rotation of the vegetal cell mass [R., Winklbauer, M., Schürfeld (1999). “Vegetal rotation, a new gastrulation movement involved in the internalization of the mesoderm and endoderm in Xenopus.” Development. 126, 3703–3713.]. We present a revised model of gastrulation movements in Xenopus laevis.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.ydbio.2007.02.005DOIArticle
http://www.sciencedirect.com/science/article/pii/S0012160607001029PublisherArticle
ORCID:
AuthorORCID
Fraser, Scott E.0000-0002-5377-0223
Jacobs, Russell E.0000-0002-1382-8486
Additional Information:© 2007 Elsevier Inc. Received for publication 11 December 2006; revised 16 January 2007; accepted 5 February 2007. Available online 13 February 2007. We thank Vladimir Korzh, Rachel S. Kraut and Rudolf Winklbauer for suggestions to improve the manuscript, Michael J. Tyszka and Seth Ruffins for technical discussion and Andrey Demyanenko for building the NMR coil. We thank Dr. Claire Corot (Guerbet Research, France) for the generous gift of the P717 contrast agent. This work was supported by the NIH Grant number HD25390. C.P. was in part supported by the Deutsche Forschungsgemeinschaft, Grant number PA 562/1-1.
Funders:
Funding AgencyGrant Number
NIHHD25390
Deutsche Forschungsgemeinschaft (DFG)PA 562/1-1
Subject Keywords:Developmental biology; Xenopus laevis; Gastrula; Spemann organizer; Longitudinal study; MRI; Contrast media
Issue or Number:1
Record Number:CaltechAUTHORS:20160121-135402120
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160121-135402120
Official Citation:Cyrus Papan, Benoit Boulat, S. Sendhil Velan, Scott E. Fraser, Russell E. Jacobs, Formation of the dorsal marginal zone in Xenopus laevis analyzed by time-lapse microscopic magnetic resonance imaging, Developmental Biology, Volume 305, Issue 1, 1 May 2007, Pages 161-171, ISSN 0012-1606, http://dx.doi.org/10.1016/j.ydbio.2007.02.005. (http://www.sciencedirect.com/science/article/pii/S0012160607001029)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63852
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:22 Jan 2016 17:18
Last Modified:03 Oct 2019 09:32

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