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Exclusive developmental functions of gatae cis-regulatory modules in the Strongylocentrorus purpuratus embryo

Lee, Pei Yun and Nam, Jongmin and Davidson, Eric H. (2007) Exclusive developmental functions of gatae cis-regulatory modules in the Strongylocentrorus purpuratus embryo. Developmental Biology, 307 (2). pp. 434-445. ISSN 0012-1606. doi:10.1016/j.ydbio.2007.05.005. https://resolver.caltech.edu/CaltechAUTHORS:20160122-095050063

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Abstract

The gatae gene of Strongylocentrotus purpuratus is orthologous to vertebrate gata-4,5,6 genes. This gene is expressed in the endomesoderm in the blastula and later the gut of the embryo, and is required for normal development. A gatae BAC containing a GFP reporter knocked into exon one of the gene was able to reproduce all aspects of endogenous gatae expression in the embryo. To identify putative gatae cis-regulatory modules we carried out an interspecific sequence conservation analysis with respect to a Lytechinus variegatus gatae BAC, which revealed 25 conserved non-coding sequence patches. These were individually tested in gene transfer experiments, and two modules capable of driving localized reporter expression in the embryo were identified. Module 10 produces early expression in mesoderm and endoderm cells up to the early gastrula stage, while module 24 generates late endodermal expression at gastrula and pluteus stages. Module 10 was then deleted from the gatae BAC by reciprocal recombination, resulting in total loss of reporter expression in the time frame in which it is normally active. Similar deletion of module 24 led to ubiquitous GFP expression in the gastrula and pluteus. These results show that Module 10 is uniquely necessary and sufficient to account for the early phase of gatae expression during endomesoderm specification. In addition, they imply a functional cis-regulatory module exclusion, whereby only a single module can associate with the basal promoter and drive gene expression at any given time.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.ydbio.2007.05.005DOIArticle
http://www.sciencedirect.com/science/article/pii/S0012160607008810PublisherArticle
Additional Information:© 2007 Elsevier Inc. Received for publication 10 February 2007; revised 1 May 2007; accepted 4 May 2007. Available online 10 May 2007. We would like to express our gratitude to Pat Leahy at Kerckhoff Marine Lab for his excellent care of the sea urchins. We would also like to thank Julie Hahn for generating the Gatae GFP BAC and for technical advice with BAC deletions. This work was supported by NIH grant HD-37105.
Funders:
Funding AgencyGrant Number
NIHHD-37105
Subject Keywords:Sea urchin; Gene regulation; GATA factors; cis-Regulatory analysis; gatae
Issue or Number:2
DOI:10.1016/j.ydbio.2007.05.005
Record Number:CaltechAUTHORS:20160122-095050063
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160122-095050063
Official Citation:Pei Yun Lee, Jongmin Nam, Eric H. Davidson, Exclusive developmental functions of gatae cis-regulatory modules in the Strongylocentrorus purpuratus embryo, Developmental Biology, Volume 307, Issue 2, 15 July 2007, Pages 434-445, ISSN 0012-1606, http://dx.doi.org/10.1016/j.ydbio.2007.05.005. (http://www.sciencedirect.com/science/article/pii/S0012160607008810)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63875
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:22 Jan 2016 20:49
Last Modified:10 Nov 2021 23:22

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