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Structure of Dengue Virus: Implications for Flavivirus Organization, Maturation, and Fusion

Kuhn, Richard J. and Zhang, Wei and Rossmann, Michael G. and Pletnev, Sergei V. and Corver, Jeroen and Lenches, Edith and Jones, Christopher T. and Mukhopadhyay, Suchetana and Chipman, Paul R. and Strauss, Ellen G. and Baker, Timothy S. and Strauss, James H. (2002) Structure of Dengue Virus: Implications for Flavivirus Organization, Maturation, and Fusion. Cell, 108 (5). pp. 717-725. ISSN 0092-8674. PMCID PMC4152842. https://resolver.caltech.edu/CaltechAUTHORS:20160125-144218251

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Abstract

The first structure of a flavivirus has been determined by using a combination of cryoelectron microscopy and fitting of the known structure of glycoprotein E into the electron density map. The virus core, within a lipid bilayer, has a less-ordered structure than the external, icosahedral scaffold of 90 glycoprotein E dimers. The three E monomers per icosahedral asymmetric unit do not have quasiequivalent symmetric environments. Difference maps indicate the location of the small membrane protein M relative to the overlaying scaffold of E dimers. The structure suggests that flaviviruses, and by analogy also alphaviruses, employ a fusion mechanism in which the distal barrels of domain II of the glycoprotein E are inserted into the cellular membrane.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0092-8674(02)00660-8DOIArticle
http://www.sciencedirect.com/science/article/pii/S0092867402006608PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4152842/PubMed CentralArticle
Additional Information:© 2002 Cell Press. Received: October 3, 2001. Revised: December 21, 2001. We thank Sharon Wilder for help in preparation of the manuscript. The work was supported by a National Institutes of Health Program Project Grant to M.G.R., R.J.K., and T.S.B. (AI45976), grant AI20612 to J.H.S., grant AI10793 to J.H.S. and E.G.S., and a Purdue University redevelopment award. C.T.J. is a recipient of a National Institutes of Health biophysics training grant (GM08296). Accession Numbers: The coordinates for dengue virus have been deposited in the Protein Data Bank with the accession number 1K4R.
Funders:
Funding AgencyGrant Number
NIHAI 45976
NIHAI20612
NIHAI10793
Purdue UniversityUNSPECIFIED
NIHGM08296
Issue or Number:5
PubMed Central ID:PMC4152842
Record Number:CaltechAUTHORS:20160125-144218251
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160125-144218251
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63942
Collection:CaltechAUTHORS
Deposited By: Donna Wrublewski
Deposited On:26 Jan 2016 18:53
Last Modified:03 Oct 2019 09:33

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