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Anchoring of Surface Proteins to the Cell Wall of Staphylococcus aureus: sortase catalyzed in vitro transpeptidation reaction using LPXTG peptide and NH2-Gly3 substrates

Ton-That, Hung and Mazmanian, Sarkis K. and Faull, Kym F. and Schneewind, Olaf (2000) Anchoring of Surface Proteins to the Cell Wall of Staphylococcus aureus: sortase catalyzed in vitro transpeptidation reaction using LPXTG peptide and NH2-Gly3 substrates. Journal of Bacteriology, 275 (13). pp. 9876-9881. ISSN 0021-9193. http://resolver.caltech.edu/CaltechAUTHORS:TONjbc00

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Abstract

Staphylococcus aureus sortase anchors surface proteins to the cell wall envelope by cleaving polypeptides at the LPXTG motif. Surface proteins are linked to the peptidoglycan by an amide bond between the C-terminal carboxyl and the amino group of the pentaglycine cross-bridge. We find that purified recombinant sortase hydrolyzed peptides bearing an LPXTG motif at the peptide bond between threonine and glycine. In the presence of NH2-Gly3, sortase catalyzed exclusively a transpeptidation reaction, linking the carboxyl group of threonine to the amino group of NH2-Gly3. In the presence of amino group donors the rate of sortase mediated cleavage at the LPXTG motif was increased. Hydrolysis and transpeptidation required the sulfhydryl of cysteine 184, suggesting that sortase catalyzed the transpeptidation reaction of surface protein anchoring via the formation of a thioester acyl-enzyme intermediate.


Item Type:Article
ORCID:
AuthorORCID
Mazmanian, Sarkis K.0000-0003-2713-1513
Additional Information:Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc. (Received for publication, December 7, 1999) This work was supported by United States Public Health Service Grant AI 38897.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)AI38897
Record Number:CaltechAUTHORS:TONjbc00
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:TONjbc00
Alternative URL:http://www.jbc.org/cgi/content/abstract/275/13/9876
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6398
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:07 Dec 2006
Last Modified:15 Oct 2015 22:44

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