CaltechAUTHORS
  A Caltech Library Service

Binding of cis- and trans-dichlorodiammineplatinum(II) to DNA: evidence for unwinding and shortening of the double helix

Cohen, Gary L. and Bauer, William R. and Barton, Jacqueline K. and Lippard, Stephen J. (1979) Binding of cis- and trans-dichlorodiammineplatinum(II) to DNA: evidence for unwinding and shortening of the double helix. Science, 203 (4384). pp. 1014-1016. ISSN 0036-8075. http://resolver.caltech.edu/CaltechAUTHORS:20160126-165802210

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:20160126-165802210

Abstract

The antitumor drug cis-dichlorodiammineplatinum(II) (cis-DDP) and the inactive trans isomer bind and produce cooperative changes in closed and nicked circular duplex DNA's. Covalent binding of both platinum complexes to the closed circular DNA alters the degree of supercoiling, presumably by disrupting and unwinding the double helix. Electron micrographs show the platinated DNA's to be shortened by up to 50 percent of their original length. At similar ratios of bound platinum per nucleotide, the electrophoretic mobilities of the DNA's in gels containing the dye ethidium bromide are the same for both isomers. The only detectable difference in the binding of the two platinum isomers is an increase in the electrophoretic mobility in nondye gels of closed circular DNA having small amounts of bound cis-DDP that is not apparent for the trans complex.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.370979DOIArticle
http://science.sciencemag.org/content/203/4384/1014.abstractPublisherArticle
ORCID:
AuthorORCID
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 1979 American Association for the Advancement of Science. 21 September 1978; revised 18 December 1978. Supported by PHS grants CA-15826 from the National Cancer Institute (to S.J.L.) and GM-21176 from the National Institute of General Medical Sciences (to W.R.B.) and by NSF grant PCM76-01839 (to W.R.B.). J.K.B. thanks the National Science Foundation for a predoctoral fellowship. A generous gift of K_2PtCl_4 from Engelhard Industries used to prepare cis- and trans-DDP is gratefully acknowledged.
Funders:
Funding AgencyGrant Number
National Cancer InstituteCA-15826
National Institute of General Medical SciencesGM-21176
NSFPCM76-01839
NSF Predoctoral FellowshipUNSPECIFIED
Record Number:CaltechAUTHORS:20160126-165802210
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160126-165802210
Official Citation:Binding of cis- and trans-dichlorodiammineplatinum(II) to DNA: evidence for unwinding and shortening of the double helix BY GL COHEN, WR BAUER, JK BARTON, SJ LIPPARD SCIENCE09 MAR 1979 : 1014-1016
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:63989
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:27 Jan 2016 19:19
Last Modified:13 Feb 2019 18:06

Repository Staff Only: item control page