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DNA repair glycosylases with a [4Fe–4S] cluster: A redox cofactor for DNA-mediated charge transport?

Boal, Amie K. and Yavin, Eylon and Barton, Jacqueline K. (2007) DNA repair glycosylases with a [4Fe–4S] cluster: A redox cofactor for DNA-mediated charge transport? Journal of Inorganic Biochemistry, 101 (11-12). pp. 1913-1921. ISSN 0162-0134. PMCID PMC2094209. doi:10.1016/j.jinorgbio.2007.05.001.

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The [4Fe–4S] cluster is ubiquitous to a class of base excision repair enzymes in organisms ranging from bacteria to man and was first considered as a structural element, owing to its redox stability under physiological conditions. When studied bound to DNA, two of these repair proteins (MutY and Endonuclease III from Escherichia coli) display DNA-dependent reversible electron transfer with characteristics typical of high potential iron proteins. These results have inspired a reexamination of the role of the [4Fe–4S] cluster in this class of enzymes. Might the [4Fe–4S] cluster be used as a redox cofactor to search for damaged sites using DNA-mediated charge transport, a process well known to be highly sensitive to lesions and mismatched bases? Described here are experiments demonstrating the utility of DNA-mediated charge transport in characterizing these DNA-binding metalloproteins, as well as efforts to elucidate this new function for DNA as an electronic signaling medium among the proteins.

Item Type:Article
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URLURL TypeDescription CentralArticle
Boal, Amie K.0000-0002-1234-8472
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2007 Elsevier Inc. Received 9 March 2007; received in revised form 30 April 2007; accepted 3 May 2007. Available online 17 May 2007. In memory of Edward I. Stiefel. We are grateful to the NIH (GM49216) for their financial support. We also thank our coworkers and collaborators for their efforts, and we are grateful to Ed Stiefel for getting one of us (J.K.B.) excited about Fe–S clusters at the beginning.
Funding AgencyGrant Number
Subject Keywords:DNA damage; DNA repair
Issue or Number:11-12
PubMed Central ID:PMC2094209
Record Number:CaltechAUTHORS:20160127-111126513
Persistent URL:
Official Citation:Amie K. Boal, Eylon Yavin, Jacqueline K. Barton, DNA repair glycosylases with a [4Fe–4S] cluster: A redox cofactor for DNA-mediated charge transport?, Journal of Inorganic Biochemistry, Volume 101, Issues 11–12, November 2007, Pages 1913-1921, ISSN 0162-0134, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64007
Deposited By: Ruth Sustaita
Deposited On:27 Jan 2016 20:05
Last Modified:10 Nov 2021 23:24

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