CaltechAUTHORS
  A Caltech Library Service

Damage to DNA by long-range charge transport

Núñez, Megan E. and Rajski, Scott R. and Barton, Jacqueline K. (2000) Damage to DNA by long-range charge transport. Methods in Enzymology, 319 . pp. 165-188. ISSN 0076-6879. http://resolver.caltech.edu/CaltechAUTHORS:20160127-111127012

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:20160127-111127012

Abstract

Photochemical reactions on DNA assemblies containing tethered photooxidants, particularly metallointercalating photooxidants, have been critical in establishing that permanent damage to DNA bases can be generated as a result of radical migration from a remote site on the DNA duplex. Induction of a 1-electron deficiency in the oxidant attached covalently to the DNA remote from the oxidizable site leads to this “chemistry at a distance,” caused by efficient charge transport through the DNA base pair stack. Double helical DNA may be unique as a polymeric assembly in solution because of this interior core of stacked aromatic heterocyclic base pairs. Similarly stacked solid-state materials tend to be conducting along the stacking direction. This chapter describes the design and construction of DNA assemblies used to probe long-range oxidative damage in DNA. It also includes methodology for the oxidative repair of a thymine dimer lesion in DNA, as this “chemistry at a distance” also depends on long-range charge transfer through the DNA base pair stack.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0076-6879(00)19018-4DOIArticle
http://www.sciencedirect.com/science/article/pii/S0076687900190184PublisherArticle
ORCID:
AuthorORCID
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2000 Academic Press. We are grateful to the NIH (GM49216) for their financial support of this research. We also thank the American Cancer Society for a postdoctoral fellowship (to S.R.R.) and the Howard Hughes Medical Institute for a predoctoral fellowship (to M.E.N.). Most of all we thank the many talented scientists whose work contributed to that described here, especially Drs. D. B. Hall, M. R. Arkin, R. E. Holmlin, and P. J. Dandliker.
Funders:
Funding AgencyGrant Number
NIHGM49216
American Cancer SocietyUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Record Number:CaltechAUTHORS:20160127-111127012
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160127-111127012
Official Citation:Megan E. Núñez, Scott R. Rajski, Jacqueline K. Barton, [16] Damage to DNA by long-range charge transport, Methods in Enzymology, Academic Press, 2000, Volume 319, Pages 165-188, ISSN 0076-6879, ISBN 9780121822200, http://dx.doi.org/10.1016/S0076-6879(00)19018-4. (http://www.sciencedirect.com/science/article/pii/S0076687900190184)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64009
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:27 Jan 2016 20:09
Last Modified:27 Jan 2016 20:09

Repository Staff Only: item control page