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Detection of DNA Base Mismatches Using DNA Intercalators

Boon, Elizabeth M. and Kisko, Jennifer L. and Barton, Jacqueline K. (2002) Detection of DNA Base Mismatches Using DNA Intercalators. Methods in Enzymology, 353 . pp. 506-522. ISSN 0076-6879. doi:10.1016/S0076-6879(02)53073-1. https://resolver.caltech.edu/CaltechAUTHORS:20160127-120857206

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Abstract

The most prevalent mechanisms leading to mutations in DNA are direct mis-incorporation of bases during replication and sustained chemical damage. Under normal circumstances, the cell corrects these problems using DNA polymerase proofreading mechanisms as well as the complex repair machinery of the cell. In certain tissues that contain mismatch repair deficiencies, DNA mispairs may accumulate.1, 2, 3, 4 and 5 Even in healthy cells, however, mismatches and lesions can sometimes go unchecked, resulting in permanent alterations in the gene sequence for subsequent generations. Identification of genetic variations (single-nucleotide polymorphisms, SNPs) among individuals and populations has implications in understanding human disease and treatment, as well as the interaction of the environment and multiple genes during evolution.6 Once these SNPs are identified and understood, rapid and reliable detection of them will be critical for the study, diagnosis, and treatment of genetically linked disease.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0076-6879(02)53073-1DOIArticle
http://www.sciencedirect.com/science/article/pii/S0076687902530731PublisherArticle
ORCID:
AuthorORCID
Boon, Elizabeth M.0000-0003-1891-839X
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2002, Elsevier Science.
DOI:10.1016/S0076-6879(02)53073-1
Record Number:CaltechAUTHORS:20160127-120857206
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160127-120857206
Official Citation:Elizabeth M. Boon, Jennifer L. Kisko, Jacqueline K. Barton, [43] - Detection of DNA Base Mismatches Using DNA Intercalators, In: Chandan K. Sen and Lester Packer, Editor(s), Methods in Enzymology, Academic Press, 2002, Volume 353, Pages 506-522, ISSN 0076-6879, ISBN 9780121822569, http://dx.doi.org/10.1016/S0076-6879(02)53073-1. (http://www.sciencedirect.com/science/article/pii/S0076687902530731)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64016
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:27 Jan 2016 22:47
Last Modified:10 Nov 2021 23:24

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