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Calcium signaling during convergent extension in Xenopus

Wallingford, John B. and Ewald, Andrew J. and Harland, Richard M. and Fraser, Scott E. (2001) Calcium signaling during convergent extension in Xenopus. Current Biology, 11 (9). pp. 652-661. ISSN 0960-9822. https://resolver.caltech.edu/CaltechAUTHORS:20160127-153937372

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Abstract

Background: During Xenopus gastrulation, cell intercalation drives convergent extension of dorsal tissues. This process requires the coordination of motility throughout a large population of cells. The signaling mechanisms that regulate these movements in space and time remain poorly understood. Results: To investigate the potential contribution of calcium signaling to the control of morphogenetic movements, we visualized calcium dynamics during convergent extension using a calcium-sensitive fluorescent dye and a novel confocal microscopy system. We found that dramatic intercellular waves of calcium mobilization occurred in cells undergoing convergent extension in explants of gastrulating Xenopus embryos. These waves arose stochastically with respect to timing and position within the dorsal tissues. Waves propagated quickly and were often accompanied by a wave of contraction within the tissue. Calcium waves were not observed in explants of the ventral marginal zone or prospective epidermis. Pharmacological depletion of intracellular calcium stores abolished the calcium dynamics and also inhibited convergent extension without affecting cell fate. These data indicate that calcium signaling plays a direct role in the coordination of convergent extension cell movements. Conclusions: The data presented here indicate that intercellular calcium signaling plays an important role in vertebrate convergent extension. We suggest that calcium waves may represent a widely used mechanism by which large groups of cells can coordinate complex cell movements.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0960-9822(01)00201-9DOIArticle
http://www.sciencedirect.com/science/article/pii/S0960982201002019PublisherArticle
ORCID:
AuthorORCID
Fraser, Scott E.0000-0002-5377-0223
Additional Information:© 2001 Elsevier Science Ltd. Received 6 February 2001, Revised 15 March 2001, Accepted 16 March 2001, Available online 7 May 2001. The authors thank C. LaBonne, J. Home, D. Koos, and E. Gasey for helpful discussions. J.B.W. is supported by the American Cancer Society (PF-99-350-01-DDC). A.J.E. is a participant in the Caltech Initiative in Computational Molecular Biology, which is funded by a Burroughs Wellcome Fund Interfaces award. This work was supported by the Beckman Institute and the National Institutes of Health.
Funders:
Funding AgencyGrant Number
American Cancer SocietyPF-99-350-01-DDC
Burroughs Wellcome FundUNSPECIFIED
Caltech Beckman InstituteUNSPECIFIED
NIHUNSPECIFIED
Issue or Number:9
Record Number:CaltechAUTHORS:20160127-153937372
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160127-153937372
Official Citation:John B. Wallingford, Andrew J. Ewald, Richard M. Harland, Scott E. Fraser, Calcium signaling during convergent extension in Xenopus, Current Biology, Volume 11, Issue 9, 1 May 2001, Pages 652-661, ISSN 0960-9822, http://dx.doi.org/10.1016/S0960-9822(01)00201-9. (http://www.sciencedirect.com/science/article/pii/S0960982201002019)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64026
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:28 Jan 2016 17:32
Last Modified:03 Oct 2019 09:34

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