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Oxidative Charge Transport through DNA in Nucleosome Core Particles

Núñez, Megan E. and Noyes, Katherine T. and Barton, Jacqueline K. (2002) Oxidative Charge Transport through DNA in Nucleosome Core Particles. Chemistry and Biology, 9 (4). pp. 403-415. ISSN 1074-5521. https://resolver.caltech.edu/CaltechAUTHORS:20160129-080804224

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Abstract

Eukaryotic DNA is packaged into nucleosomes, made up of 146 bp of DNA wrapped around a core of histone proteins. We used photoexcited rhodium intercalators to explore DNA charge transport within these assemblies. Although histone proteins inhibit intercalation of the rhodium complex within the core particle, they do not prevent 5′-GG-3′ oxidation, the signature of oxidative charge transport through DNA. Moreover, using rhodium intercalators tethered to the 5′ terminus of the DNA, we found that guanine bases within the nucleosome can be oxidized from a distance of 24 bp. Histone binding did not affect the pattern and extent of this oxidation. Therefore, although the structure of the nucleosome core particle generally protects DNA from damage by solution-borne molecules, packaging within the nucleosome does not protect DNA from charge transfer damage through the base pair stack.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S1074-5521(02)00121-7DOIArticle
http://www.sciencedirect.com/science/article/pii/S1074552102001217PublisherArticle
ORCID:
AuthorORCID
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2002 Elsevier Science Ltd. Received 30 October 2001, Revised 7 January 2002, Accepted 11 January 2002, Available online 22 April 2002. This work was supported by the National Institutes of Health (GM49216), the National Foundation for Cancer Research, the Howard Hughes Medical Institute predoctoral fellowship program (M.E.N.), and the Caltech SURF program (K.T.N.). We would like to thank Karolin Luger and Armin W. Mäder for advice and protocols regarding the preparation of the palindromic 146-mer.
Funders:
Funding AgencyGrant Number
NIHGM49216
National Foundation for Cancer ResearchUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Caltech Summer Undergraduate Research Fellowship (SURF)UNSPECIFIED
Issue or Number:4
Record Number:CaltechAUTHORS:20160129-080804224
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160129-080804224
Official Citation:Megan E Núñez, Katherine T Noyes, Jacqueline K Barton, Oxidative Charge Transport through DNA in Nucleosome Core Particles, Chemistry & Biology, Volume 9, Issue 4, April 2002, Pages 403-415, ISSN 1074-5521, http://dx.doi.org/10.1016/S1074-5521(02)00121-7. (http://www.sciencedirect.com/science/article/pii/S1074552102001217)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64080
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:29 Jan 2016 17:09
Last Modified:03 Oct 2019 09:34

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