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A single-stranded deoxyribonucleic acid from bacteriophage φX174

Sinsheimer, Robert L. (1959) A single-stranded deoxyribonucleic acid from bacteriophage φX174. Journal of Molecular Biology, 1 (1). pp. 43-53. ISSN 0022-2836. https://resolver.caltech.edu/CaltechAUTHORS:20160129-143614434

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Abstract

The deoxyribonucleic acid (DNA) of bacteriophage φX174 can be extracted by phenolic denaturation of the virus protein. The DNA thus obtained has a molecular weight of 1·7 × 10^6, indicating that there is one molecule per virus particle. This φX DNA does not have a complementary nucleotide composition. The ultraviolet absorption of this DNA is strongly dependent upon temperature in the range 20° to 60°C, and upon NaCl concentration in the range 10^(−3) to 10^0 M. This DNA reacts with formaldehyde at 37°C and is precipitated by plumbous ions. This evidence is interpreted to mean that the purine and pyrimidine rings are not involved in a tightly hydrogen-bonded complementary structure. Light scattering studies indicate that this DNA is highly flexible and that its configuration is strongly dependent upon the ionic strength of the solution. Upon treatment with pancreatic deoxyribonuclease, the weight-average molecular weight decreases in accordance with the function expected for a single-stranded molecule. It is concluded that the DNA of bacteriophage φX174 is single-stranded.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0022-2836(59)80006-1DOIArticle
http://www.sciencedirect.com/science/article/pii/S0022283659800061PublisherArticle
Additional Information:© 1959 Elsevier Ltd. (Received 16 January 1959) This research has been supported by grants from the U.S. Public Health Service, the American Cancer Society, and the California Division of the American Cancer Society.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)UNSPECIFIED
American Cancer SocietyUNSPECIFIED
American Cancer Society, California DivisionUNSPECIFIED
Issue or Number:1
Record Number:CaltechAUTHORS:20160129-143614434
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160129-143614434
Official Citation:Robert L. Sinsheimer, A single-stranded deoxyribonucleic acid from bacteriophage φX174, Journal of Molecular Biology, Volume 1, Issue 1, 1959, Pages 43-IN6, ISSN 0022-2836, http://dx.doi.org/10.1016/S0022-2836(59)80006-1. (http://www.sciencedirect.com/science/article/pii/S0022283659800061)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64101
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:02 Feb 2016 20:37
Last Modified:03 Oct 2019 09:34

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