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Migratory patterns of cloned neural crest melanocytes injected into host chicken embryos

Bronner, Marianne E. and Cohen, Alan M. (1979) Migratory patterns of cloned neural crest melanocytes injected into host chicken embryos. Proceedings of the National Academy of Sciences of the United States of America, 76 (4). pp. 1843-1847. ISSN 0027-8424. PMCID PMC383488. http://resolver.caltech.edu/CaltechAUTHORS:20160129-144855016

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Abstract

Cloned quail melanocytes grown in tissue culture for 8 days or more were injected into 2 1/2-day-old chicken embryos. The pigment cells were placed directly into the somitic lumen by means of an injection micropipette. This technique for introducing marked neural crest cells into host embryos causes far less damage than previous methods which require extirpation and replacement of the neural tube. In addition, small numbers of homogeneous cells can be implanted by this procedure. When injected into one of the posterior somites, cultured pigment cells migrated along the ventral neural crest pathway. Three days after injection the melanocytes had migrated ventral to the dorsal root ganglia and prevertebral and primary sympathetic chain ganglia and were seen associated with the adrenal gland and aortic plexi. Melanocytes were frequently found in or adjacent to the gonads and often had migrated as far as the gut.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.pnas.org/content/76/4/1843PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC383488/PubMed CentralArticle
ORCID:
AuthorORCID
Bronner, Marianne E.0000-0003-4274-1862
Additional Information:© 1979 National Academy of Sciences. Communicated by David Bodian, January 15, 1979. The research was supported by U.S. Public Health Service Grant HD-07389 and a Basic Research Grant from The National Foundation-March of Dimes awarded to A.M.C. and U.S. Public Health Service Predoctoral Training Grant 5T32GM07231 to M.E.B. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)HD-07389
National March of DimesUNSPECIFIED
NIH Predoctoral Fellowship5T32GM07231
Subject Keywords:migration; pigment cells; injection technique; differentiation
PubMed Central ID:PMC383488
Record Number:CaltechAUTHORS:20160129-144855016
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160129-144855016
Official Citation:M E Bronner and A M Cohen Migratory patterns of cloned neural crest melanocytes injected into host chicken embryos PNAS 1979 76 (4) 1843-1847
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64103
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:01 Feb 2016 16:07
Last Modified:13 Feb 2019 18:06

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