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Dengue 2 Virus NS2B and NS3 Form a Stable Complex That Can Cleave NS3 within the Helicase Domain

Arias, Carlos F. and Preugschat, Frank and Strauss, James H. (1993) Dengue 2 Virus NS2B and NS3 Form a Stable Complex That Can Cleave NS3 within the Helicase Domain. Virology, 193 (2). pp. 888-899. ISSN 0042-6822. https://resolver.caltech.edu/CaltechAUTHORS:20160203-144803611

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Abstract

Flavivirus genomic RNA is translated into a large polyprotein that is proceSsed into structural and nonstructural proteins. The N-termini of several nonstructural proteins are produced by cleavage at dibasic sites by a two-component viral proteinase consisting of NS2B and NS3. NS3 contains a trypsin-like serine proteinase domain at its N-terminus, whereas the function of NS2B in proteolysis is yet to be determined. We have used an NS3-specific antiserum, under nondenaturing conditions, to demonstrate that NS2B and NS3 form a complex both in vitro and in vivo. The N-terminal 184 residues of NS3 are sufficient to form the complex with NS28. The complex forms efficiently when the NS2B and NS3 are translated from two different 1nRNAs as well as when NS28 and NS3 are translated as a polyprotein from the same mRNA. A chimeric complex can be formed between yellow fever NS2B and a chimeric yellow fever-dengue 2 NS3. Using anti-NS3 antisera, we also found that a 50-kDa fragment of NS3, consisting of the N-terminal approximately 460 residues, is produced in infected mammalian cells. This fragment is not produced in infected mosquito cells, but will form in Triton X-100 lysates of mosquito cells. The cleavage of NS3 to form this fragment is catalyzed by the NS3 proteinase itself and proteolysis requires NS28. Examination of the amino acid sequence of NS3 reveals a potential conserved cleavage site that resembles other sites cleaved by the NS3/NS2B proteinase; this site occurs within a conserved RNA helicase sequence motif. The importance of this alternatively processed form of NS3 and its role in the replication cycle of dengue virus remain to be determined.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1006/viro.1993.1198DOIArticle
http://www.sciencedirect.com/science/article/pii/S0042682283711980PublisherArticle
Additional Information:© 1993 Academic Press, Inc. Received October 28, 1992; accepted December 14, 1992. The authors thank Ellen Strauss for stimulating discussions and editorial expertise. This work was supported by Grant AI20612 from the National Institutes of Health and by Grant V22/181/10 from the World Health Organization.
Funders:
Funding AgencyGrant Number
NIHAI20612
World Health OrganizationV22/181/10
Issue or Number:2
Record Number:CaltechAUTHORS:20160203-144803611
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160203-144803611
Official Citation:Carlos F. Arias, Frank Preugschat, James H. Strauss, Dengue 2 Virus NS2B and NS3 Form a Stable Complex That Can Cleave NS3 within the Helicase Domain, Virology, Volume 193, Issue 2, April 1993, Pages 888-899, ISSN 0042-6822, http://dx.doi.org/10.1006/viro.1993.1198. (http://www.sciencedirect.com/science/article/pii/S0042682283711980)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64206
Collection:CaltechAUTHORS
Deposited By: Donna Wrublewski
Deposited On:04 Feb 2016 00:11
Last Modified:03 Oct 2019 09:35

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